Hosuk:LabNotes/2013-6-23: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===Rolony, 11th=== *S3 --> 3uL, S4 --> 10uL of Phi29 *Use new vial of BS(PEG9) bought at 06/05 *Start at 06/19 *Use cell dishes fixed at 06/19 ...") |
>Hosuki78 No edit summary |
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*Run RT : 9:00pm 06/23 ~ 9:00am 06/24 --> ~12hr. | *Run RT : 9:00pm 06/23 ~ 9:00am 06/24 --> ~12hr. | ||
*Run CircLigase II : 1:00am 06/24 ~ 5:00pm 06/24 --> 4hr. | *Run CircLigase II : 1:00am 06/24 ~ 5:00pm 06/24 --> 4hr. | ||
*Run RCA : 6:10 pm 06/24 ~ 2: 10pm 06/ | *Run RCA : 6:10 pm 06/24 ~ 2: 10pm 06/25 --> 20hr. | ||
====Result==== | ====Result==== | ||
* | *'''SUCCESS!!''' |
Latest revision as of 00:55, 26 June 2013
Rolony, 11th[edit]
- S3 --> 3uL, S4 --> 10uL of Phi29
- Use new vial of BS(PEG9) bought at 06/05
- Start at 06/19
- Use cell dishes fixed at 06/19
- Fixing cells with 10% Formalin once (like Jonathan's cell dish)
- Run RT : 9:00pm 06/23 ~ 9:00am 06/24 --> ~12hr.
- Run CircLigase II : 1:00am 06/24 ~ 5:00pm 06/24 --> 4hr.
- Run RCA : 6:10 pm 06/24 ~ 2: 10pm 06/25 --> 20hr.
Result[edit]
- SUCCESS!!