Hosuk:LabNotes/2013-6-23: Difference between revisions

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>Hosuki78
(Created page with "*LabNote ===Rolony, 11th=== *S3 --> 3uL, S4 --> 10uL of Phi29 *Use new vial of BS(PEG9) bought at 06/05 *Start at 06/19 *Use cell dishes fixed at 06/19 ...")
 
>Hosuki78
No edit summary
 
Line 13: Line 13:
*Run RT : 9:00pm 06/23 ~ 9:00am 06/24 --> ~12hr.
*Run RT : 9:00pm 06/23 ~ 9:00am 06/24 --> ~12hr.
*Run CircLigase II : 1:00am 06/24 ~ 5:00pm 06/24  --> 4hr.
*Run CircLigase II : 1:00am 06/24 ~ 5:00pm 06/24  --> 4hr.
*Run RCA : 6:10 pm 06/24 ~ 2: 10pm 06/24 --> 20hr.
*Run RCA : 6:10 pm 06/24 ~ 2: 10pm 06/25 --> 20hr.




====Result====
====Result====
*...
*'''SUCCESS!!'''

Latest revision as of 00:55, 26 June 2013

Rolony, 11th[edit]

  • S3 --> 3uL, S4 --> 10uL of Phi29
  • Use new vial of BS(PEG9) bought at 06/05


  • Start at 06/19
  • Use cell dishes fixed at 06/19
  • Fixing cells with 10% Formalin once (like Jonathan's cell dish)


  • Run RT : 9:00pm 06/23 ~ 9:00am 06/24 --> ~12hr.
  • Run CircLigase II : 1:00am 06/24 ~ 5:00pm 06/24 --> 4hr.
  • Run RCA : 6:10 pm 06/24 ~ 2: 10pm 06/25 --> 20hr.


Result[edit]

  • SUCCESS!!