Kun:LabNotes/ASE/2007-10-30: Difference between revisions

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(New page: Exp.1: Repeat PGP1Ver2 PCR on PGP1 gDNAs and Hues6 g/cDNA using NEB's 2X Taq Master mix Set up reactions on ice: One 33x Template 1ul ...)
 
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94C 2min -> 40 cycles of (94C 30S -> 57C 30S -> 68C 1min) -> 68C 3min on Chromo4
94C 2min -> 40 cycles of (94C 30S -> 57C 30S -> 68C 1min) -> 68C 3min on Chromo4
  [[Image:2007-10-31-PGP1VER2-PCR-plate2.jpg]]

Latest revision as of 17:19, 1 November 2007

Exp.1: Repeat PGP1Ver2 PCR on PGP1 gDNAs and Hues6 g/cDNA using NEB's 2X Taq Master mix

Set up reactions on ice:

                      One       33x       
  Template            1ul       33ul     
  2X Taq Mix         25ul      825ul
  50X SYBG I        0.4ul     13.2ul
  10uM Primers        1ul       each
  H2O              22.6ul      746ul

94C 2min -> 40 cycles of (94C 30S -> 57C 30S -> 68C 1min) -> 68C 3min on Chromo4

  File:2007-10-31-PGP1VER2-PCR-plate2.jpg