Hosuk:LabNotesMDA/2013-6-28: Difference between revisions

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>Hosuki78
(Created page with "*LabNote ===MDA with PGP1F, 6th=== *MDA run for 8hr @06/27 *'''400mM ALS''' --> original protocol *'''Slow mixing''' --> 1 sec period, mixing for 5min...")
 
>Hosuki78
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Line 12: Line 12:
====Verification with qPCR====
====Verification with qPCR====
*Result looked good.
*Result looked good.
*[[File:qPCR_Result_2013-06-28_Edited.png|400px]]
*[[File:qPCR_Result_MassTable_2013-06-28.png|400px]]





Revision as of 17:45, 28 June 2013

MDA with PGP1F, 6th

  • MDA run for 8hr @06/27
  • 400mM ALS --> original protocol
  • Slow mixing --> 1 sec period, mixing for 5min
  • Push ring mixer volume with air
    • Left 8 chamber were empty, too many empty chamber...


Verification with qPCR

  • Result looked good.


CoRE fragmentation

  • Done @06/28