Hosuk:LabNotesMDA/2013-6-28: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===MDA with PGP1F, 6th=== *MDA run for 8hr @06/27 *'''400mM ALS''' --> original protocol *'''Slow mixing''' --> 1 sec period, mixing for 5min...") |
>Hosuki78 No edit summary |
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====Verification with qPCR==== | ====Verification with qPCR==== | ||
*Result looked good. | *Result looked good. | ||
*[[File:qPCR_Result_2013-06-28_Edited.png|400px]] | |||
*[[File:qPCR_Result_MassTable_2013-06-28.png|400px]] | |||
Revision as of 17:45, 28 June 2013
MDA with PGP1F, 6th
- MDA run for 8hr @06/27
- 400mM ALS --> original protocol
- Slow mixing --> 1 sec period, mixing for 5min
- Push ring mixer volume with air
- Left 8 chamber were empty, too many empty chamber...
Verification with qPCR
- Result looked good.
CoRE fragmentation
- Done @06/28