Matt:LabNotes/2013-6-28: Difference between revisions
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>Mzcai (Created page with "====gDNA concentration==== Followed [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/Qubit_Protocol Qubit Protocol] to measure DNA concentration of cells (12878) ...") |
>Mzcai No edit summary |
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==== | ===Probe Capture=== | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''20 gap''' | |||
| align="center" style="background:#f0f0f0;"|'''0 gap''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Probe Size||12964||13179|| | |||
|- | |||
| gDNA HAPMAP C1 (87ng/ul)||300 ng||300 ng|| | |||
|- | |||
| gDNA MW||1.95x10^12 g/mol||1.95x10^12 g/mol|| | |||
|- | |||
| gDNA||1.5385x10^-19 mol||1.5385x10^-19 mol|| | |||
|- | |||
| Probe Required (1000:1)||1.5385x10^-16 mol||1.5385x10^-16 mol|| | |||
|- | |||
| Probe MW||8.1419x10^8 g/mol||8.2769x10^8 g/mol||(Probe size x (193nt x 325 Da/nt + 79Da) | |||
|- | |||
| Amount Probe Required||125 ng (7.44ul)||127 ng (4.47 ul)|| | |||
|- | |||
| | |||
|} | |||
===Probe | 0 gap probes from [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-6-27 2013-6-27] <br> | ||
20 gap probes from [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-6-4 2013-6-6] <br> | |||
cDNA from [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-6-26 2013-6-26] <br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Tube #''' | |||
| align="center" style="background:#f0f0f0;"|'''Probe type''' | |||
| align="center" style="background:#f0f0f0;"|'''Samples''' | |||
| align="center" style="background:#f0f0f0;"|'''Probes (ul)''' | |||
| align="center" style="background:#f0f0f0;"|'''10X AmpLigase Buffer (ul)''' | |||
| align="center" style="background:#f0f0f0;"|'''Target (ul)''' | |||
| align="center" style="background:#f0f0f0;"|'''H2O (ul)''' | |||
| align="center" style="background:#f0f0f0;"|'''Total''' | |||
|- | |||
| 1||0 gap (28.4ng/ul)||NTC||4.47||3||0||22.53||30 | |||
|- | |||
| 2||0 gap (28.4ng/ul)||gDNA HAPMAP C1 (87ng/ul)||4.47||3||3.45||19.08||30 | |||
|- | |||
| 3||0 gap (28.4ng/ul)||cDNA||4.47||3||20||2.53||30 | |||
|- | |||
| 4||20 gap (16.79ng/ul)||NTC||7.44||3||0||19.56||30 | |||
|- | |||
| 5||20 gap (16.79ng/ul)||gDNA HAPMAP C1 (87ng/ul)||7.44||3||3.45||16.11||30 | |||
|- | |||
| 6||20 gap (16.79ng/ul)||cDNA||7.44||3||20||0||30.44 | |||
|- | |||
| | |||
|} |
Revision as of 21:28, 28 June 2013
Probe Capture
' | 20 gap | 0 gap | ' |
Probe Size | 12964 | 13179 | |
gDNA HAPMAP C1 (87ng/ul) | 300 ng | 300 ng | |
gDNA MW | 1.95x10^12 g/mol | 1.95x10^12 g/mol | |
gDNA | 1.5385x10^-19 mol | 1.5385x10^-19 mol | |
Probe Required (1000:1) | 1.5385x10^-16 mol | 1.5385x10^-16 mol | |
Probe MW | 8.1419x10^8 g/mol | 8.2769x10^8 g/mol | (Probe size x (193nt x 325 Da/nt + 79Da) |
Amount Probe Required | 125 ng (7.44ul) | 127 ng (4.47 ul) | |
0 gap probes from 2013-6-27
20 gap probes from 2013-6-6
cDNA from 2013-6-26
Tube # | Probe type | Samples | Probes (ul) | 10X AmpLigase Buffer (ul) | Target (ul) | H2O (ul) | Total |
1 | 0 gap (28.4ng/ul) | NTC | 4.47 | 3 | 0 | 22.53 | 30 |
2 | 0 gap (28.4ng/ul) | gDNA HAPMAP C1 (87ng/ul) | 4.47 | 3 | 3.45 | 19.08 | 30 |
3 | 0 gap (28.4ng/ul) | cDNA | 4.47 | 3 | 20 | 2.53 | 30 |
4 | 20 gap (16.79ng/ul) | NTC | 7.44 | 3 | 0 | 19.56 | 30 |
5 | 20 gap (16.79ng/ul) | gDNA HAPMAP C1 (87ng/ul) | 7.44 | 3 | 3.45 | 16.11 | 30 |
6 | 20 gap (16.79ng/ul) | cDNA | 7.44 | 3 | 20 | 0 | 30.44 |