Hosuk:LabNotesMDA/2013-7-2: Difference between revisions

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(Created page with "*LabNote ===MDA with PGP1F, 7h=== *'''269mM ALS for 240mM ALS''' in ring mixer *Run MDA with the volume in ring mixer! --> 25th chamber *Mixing pump ...")
 
>Hosuki78
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===MDA with PGP1F, 7h===
===MDA with PGP1F, 7th===
*'''269mM ALS for 240mM ALS''' in ring mixer
*'''269mM ALS for 240mM ALS''' in ring mixer
*Run MDA with the volume in ring mixer! --> 25th chamber
*Run MDA with the volume in ring mixer! --> 25th chamber
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====Result====
====Result====
*qPCR result :
*Most of chambers have 0.5~2ng of amplicons, except Ch23, and 25
**Looked a little higher amount than 6th result
**25th chamber(Ring mixer) had very small amount
*This time, empty chamber were left 4, but they also have small remaining of DNA solution.
*It seemed that 240mM of ALS is better than 133mM.
 
 
[[File:qPCR_Result_2013-07-02_Edited.png|850px]]  [[File:qPCR_Result_AmpliconMass_2013-07-02_Table.png|200px]]
 
====CoRE fragmentation====
*Done @07/02

Latest revision as of 18:22, 26 July 2013


MDA with PGP1F, 7th[edit]

  • 269mM ALS for 240mM ALS in ring mixer
  • Run MDA with the volume in ring mixer! --> 25th chamber
  • Mixing pump : 1sec period, 5min
  • Push ring mixer volume with air
  • Verification of the amount of amplicon by qPCR
  • Start 10:00pm 07/01
  • End 09:00am 07.02 --> about 11hr


Result[edit]

  • Most of chambers have 0.5~2ng of amplicons, except Ch23, and 25
    • Looked a little higher amount than 6th result
    • 25th chamber(Ring mixer) had very small amount
  • This time, empty chamber were left 4, but they also have small remaining of DNA solution.
  • It seemed that 240mM of ALS is better than 133mM.


File:QPCR Result 2013-07-02 Edited.png File:QPCR Result AmpliconMass 2013-07-02 Table.png

CoRE fragmentation[edit]

  • Done @07/02