Hosuk:LabNotesMDA/2013-7-2: Difference between revisions
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===MDA with PGP1F, | ===MDA with PGP1F, 7th=== | ||
*'''269mM ALS for 240mM ALS''' in ring mixer | *'''269mM ALS for 240mM ALS''' in ring mixer | ||
*Run MDA with the volume in ring mixer! --> 25th chamber | *Run MDA with the volume in ring mixer! --> 25th chamber |
Latest revision as of 18:22, 26 July 2013
MDA with PGP1F, 7th[edit]
- 269mM ALS for 240mM ALS in ring mixer
- Run MDA with the volume in ring mixer! --> 25th chamber
- Mixing pump : 1sec period, 5min
- Push ring mixer volume with air
- Verification of the amount of amplicon by qPCR
- Start 10:00pm 07/01
- End 09:00am 07.02 --> about 11hr
Result[edit]
- Most of chambers have 0.5~2ng of amplicons, except Ch23, and 25
- Looked a little higher amount than 6th result
- 25th chamber(Ring mixer) had very small amount
- This time, empty chamber were left 4, but they also have small remaining of DNA solution.
- It seemed that 240mM of ALS is better than 133mM.
File:QPCR Result 2013-07-02 Edited.png File:QPCR Result AmpliconMass 2013-07-02 Table.png
CoRE fragmentation[edit]
- Done @07/02