Daniel:Protocols/CentriSep: Difference between revisions
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Princeton Separations Info: [[File:Centri-Sep_Procedure.pdf]] | Princeton Separations Info: [[File:Centri-Sep_Procedure.pdf]] | ||
Catalog No. | |||
*CS-900 (32 pack) $109 | *CS-900 (32 pack) $109 | ||
*CS-901 (100 pack) $287 | *CS-901 (100 pack) $287 |
Revision as of 22:22, 2 July 2013
Centri-Sep Protocol
Princeton Separations Info: File:Centri-Sep Procedure.pdf
Catalog No.
- CS-900 (32 pack) $109
- CS-901 (100 pack) $287
Use this protocol for Centri-Sep column purifications (Princeton Separations). This protocol uses size exclusion to separate DNA components from fluorophores post dye coupling.
Protocol
1. Hydrate in 800 uL ddH20; Allow at least 30 minutes for hydration, be sure to hydrate all the gel 2. Remove air bubbles by inverting and sharply tapping the column; stand up column and allow to settle 3. Drain the column into a wash tube (200-250 uL will drain) 4. Centrifuge at 750xg for 2 minutes (~3000 rpm; removes 300 uL fluid) 5. Transfer 20 uL reaction mixture to the top of the gel; do not disturb gel surface 6. Place column into collection tube and centrifuge at 750xg for 2 minutes 7. Dry sample in vacuum centrifuge