Daniel:Notebook/HiResChrPaint/2013-7-8: Difference between revisions

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  4. Incubate for 15 minutes at 80C, snap cool on ice
  4. Incubate for 15 minutes at 80C, snap cool on ice
  5. Purify using [[Daniel:Protocols/CentriSep|Centri-Sep Column]]
  5. Purify using [[Daniel:Protocols/CentriSep|Centri-Sep Column]]
===Results===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt"
| align="center" width="63" height="15"  valign="bottom" | &nbsp;
|style="font-weight:bold" width="65" align="center" valign="bottom" | AmpR
|style="font-weight:bold" width="65" align="center" valign="bottom" | AmpR 1
|style="font-weight:bold" width="65" align="center" valign="bottom" | AmpR 2
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="15" | ng/uL DNA
| align="center" | 538
| align="center" | 58.1
| align="center" | 51.6
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="30" | pmol/uL dye
| align="center" | 0.1
| align="center" | 1.2
| align="center" | 1.2
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="15" | ug DNA
| align="center" | 0.5
| align="center" | 1.2
| align="center" | 1.0
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="30" | pmol/uL DNA
| align="center" | 32.6
| align="center" | 3.5
| align="center" | 3.1
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="15" | dye/probe
| align="center" | 0.003
| align="center" | 0.341
| align="center" | 0.384
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="30" | labeling efficiency
| align="center" | 0.2
| align="center" | 17.0
| align="center" | 19.2
|}

Revision as of 20:37, 8 July 2013

S2 Probe Prep (Started 5/29/2013)

Back to Calendar

ULYSIS Dye Coupling (Test)

Going to use the probe Noi gave me a while back to test yield of ULYSIS dye coupling.  Going to try two for better confidence.
1. Add 1 uL DNA in 19 uL labeling buffer (Component C)
2. Denature DNA for 5 minutes at 95C; snap cool on ice
3. Add 1 uL dye to sample; add 4 uL labeling buffer
4. Incubate for 15 minutes at 80C, snap cool on ice
5. Purify using Centri-Sep Column

Results

  AmpR AmpR 1 AmpR 2
ng/uL DNA 538 58.1 51.6
pmol/uL dye 0.1 1.2 1.2
ug DNA 0.5 1.2 1.0
pmol/uL DNA 32.6 3.5 3.1
dye/probe 0.003 0.341 0.384
labeling efficiency 0.2 17.0 19.2