Daniel:Notebook/HiResChrPaint/2013-7-8: Difference between revisions
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4. Incubate for 15 minutes at 80C, snap cool on ice | 4. Incubate for 15 minutes at 80C, snap cool on ice | ||
5. Purify using [[Daniel:Protocols/CentriSep|Centri-Sep Column]] | 5. Purify using [[Daniel:Protocols/CentriSep|Centri-Sep Column]] | ||
===Results=== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt" | |||
| align="center" width="63" height="15" valign="bottom" | | |||
|style="font-weight:bold" width="65" align="center" valign="bottom" | AmpR | |||
|style="font-weight:bold" width="65" align="center" valign="bottom" | AmpR 1 | |||
|style="font-weight:bold" width="65" align="center" valign="bottom" | AmpR 2 | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
|style="font-weight:bold" height="15" | ng/uL DNA | |||
| align="center" | 538 | |||
| align="center" | 58.1 | |||
| align="center" | 51.6 | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
|style="font-weight:bold" height="30" | pmol/uL dye | |||
| align="center" | 0.1 | |||
| align="center" | 1.2 | |||
| align="center" | 1.2 | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
|style="font-weight:bold" height="15" | ug DNA | |||
| align="center" | 0.5 | |||
| align="center" | 1.2 | |||
| align="center" | 1.0 | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
|style="font-weight:bold" height="30" | pmol/uL DNA | |||
| align="center" | 32.6 | |||
| align="center" | 3.5 | |||
| align="center" | 3.1 | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
|style="font-weight:bold" height="15" | dye/probe | |||
| align="center" | 0.003 | |||
| align="center" | 0.341 | |||
| align="center" | 0.384 | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
|style="font-weight:bold" height="30" | labeling efficiency | |||
| align="center" | 0.2 | |||
| align="center" | 17.0 | |||
| align="center" | 19.2 | |||
|} |
Revision as of 20:37, 8 July 2013
S2 Probe Prep (Started 5/29/2013)
ULYSIS Dye Coupling (Test)
Going to use the probe Noi gave me a while back to test yield of ULYSIS dye coupling. Going to try two for better confidence.
- Following ULYSIS protocol
1. Add 1 uL DNA in 19 uL labeling buffer (Component C) 2. Denature DNA for 5 minutes at 95C; snap cool on ice 3. Add 1 uL dye to sample; add 4 uL labeling buffer 4. Incubate for 15 minutes at 80C, snap cool on ice 5. Purify using Centri-Sep Column
Results
AmpR | AmpR 1 | AmpR 2 | |
ng/uL DNA | 538 | 58.1 | 51.6 |
pmol/uL dye | 0.1 | 1.2 | 1.2 |
ug DNA | 0.5 | 1.2 | 1.0 |
pmol/uL DNA | 32.6 | 3.5 | 3.1 |
dye/probe | 0.003 | 0.341 | 0.384 |
labeling efficiency | 0.2 | 17.0 | 19.2 |