Daniel:Notebook/HiResChrPaint/2013-7-11: Difference between revisions
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>Djacobse (Created page with "=S2 Probe Prep (Started 5/29/2013)= Back to Calendar ==ULYSIS Dye Coupling (Test 6)== Should ...") |
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*Following [[Daniel:Protocols/AlexaDyeCouple#ULYSIS|ULYSIS protocol]] | *Following [[Daniel:Protocols/AlexaDyeCouple#ULYSIS|ULYSIS protocol]] | ||
1 | 1. Add 1 uL DNA in 19 uL labeling buffer (Component C) | ||
2. Denature DNA for 5 minutes at 95C; snap cool on ice | |||
3. Add 1 uL dye to sample; add 4 uL labeling buffer | |||
4. Incubate for 15 minutes (AmpR9) or 20 minutes (AmpR10) at 80C, snap cool on ice | |||
5. Purify using [[Daniel:Protocols/CentriSep|Centri-Sep Column]] | |||
Revision as of 18:25, 11 July 2013
S2 Probe Prep (Started 5/29/2013)
ULYSIS Dye Coupling (Test 6)
Should be the last test. I'm going to test at 15 minutes and 20 minutes incubation time, 1 uL each. If the results go well this morning I will be labeling the V6S2 set this afternoon.
- Following ULYSIS protocol
1. Add 1 uL DNA in 19 uL labeling buffer (Component C) 2. Denature DNA for 5 minutes at 95C; snap cool on ice 3. Add 1 uL dye to sample; add 4 uL labeling buffer 4. Incubate for 15 minutes (AmpR9) or 20 minutes (AmpR10) at 80C, snap cool on ice 5. Purify using Centri-Sep Column