Daniel:Notebook/HiResChrPaint/2013-7-11: Difference between revisions

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*Following [[Daniel:Protocols/AlexaDyeCouple#ULYSIS|ULYSIS protocol]]
*Following [[Daniel:Protocols/AlexaDyeCouple#ULYSIS|ULYSIS protocol]]


  1. Add 5 uL DMSO to Alexa dye 488
  1. Add 1 uL DNA in 19 uL labeling buffer (Component C)
2. Add 1 uL DNA in 19 uL labeling buffer (Component C)
  2. Denature DNA for 5 minutes at 95C; snap cool on ice
  3. Denature DNA for 5 minutes at 95C; snap cool on ice
  3. Add 1 uL dye to sample; add 4 uL labeling buffer
  4. Add 1 uL dye to sample; add 4 uL labeling buffer
  4. Incubate for 15 minutes (AmpR9) or 20 minutes (AmpR10) at 80C, snap cool on ice
  5. Incubate for 20 minutes at 80C, snap cool on ice
  5. Purify using [[Daniel:Protocols/CentriSep|Centri-Sep Column]]
  6. Measure concentrations in Nanodrop
7. Purify using [[Daniel:Protocols/CentriSep|Centri-Sep Column]]

Revision as of 18:25, 11 July 2013

S2 Probe Prep (Started 5/29/2013)

Back to Calendar

ULYSIS Dye Coupling (Test 6)

Should be the last test.  I'm going to test at 15 minutes and 20 minutes incubation time, 1 uL each.  If the
results go well this morning I will be labeling the V6S2 set this afternoon. 
1. Add 1 uL DNA in 19 uL labeling buffer (Component C)
2. Denature DNA for 5 minutes at 95C; snap cool on ice
3. Add 1 uL dye to sample; add 4 uL labeling buffer
4. Incubate for 15 minutes (AmpR9) or 20 minutes (AmpR10) at 80C, snap cool on ice
5. Purify using Centri-Sep Column