Daniel:Notebook/HiResChrPaint/2013-7-17: Difference between revisions
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===DpnII Digest gel=== | ===DpnII Digest gel=== | ||
1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample for all) | 1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all) | ||
{{GelLanes|Lane3=25bp ladder|Lane4=V4S3A}} | {{GelLanes|Lane3=25bp ladder|Lane4=V4S3A|Lane5=V4S3B|Lane6=V4S3C|Lane7=V4S3D|Lane8=V6S3A|Lane9=V6S3B|Lane10=V6S3C|Lane11=V6S3D}} | ||
2. Run for 25 minutes at 250V | |||
'''Gel Image''' | |||
==Lambda Exonuclease Digestion== |
Revision as of 18:33, 17 July 2013
S3 Probe Preparation (Started 7/16/2013)
DpnII Digestion
3. Column Purification, elute with 30 uL EB 4. Measure concentrations in nanodrop
DpnII Digest gel
1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)
Lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
Sample | 25bp ladder | V4S3A | V4S3B | V4S3C | V4S3D | V6S3A | V6S3B | V6S3C | V6S3D |
2. Run for 25 minutes at 250V
Gel Image