Daniel:Notebook/HiResChrPaint/2013-7-17: Difference between revisions

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===DpnII Digest gel===
===DpnII Digest gel===


  1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample for all)
  1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)


{{GelLanes|Lane3=25bp ladder|Lane4=V4S3A}}
{{GelLanes|Lane3=25bp ladder|Lane4=V4S3A|Lane5=V4S3B|Lane6=V4S3C|Lane7=V4S3D|Lane8=V6S3A|Lane9=V6S3B|Lane10=V6S3C|Lane11=V6S3D}}
2. Run for 25 minutes at 250V
 
'''Gel Image'''
 
==Lambda Exonuclease Digestion==

Revision as of 18:33, 17 July 2013

S3 Probe Preparation (Started 7/16/2013)

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DpnII Digestion

3. Column Purification, elute with 30 uL EB
4. Measure concentrations in nanodrop

DpnII Digest gel

1. Lanes; (5.5 uL TBE; 2 uL 6x buffer, 0.5 uL sample; load 7 uL for all)


Lane 1 2 3 4 5 6 7 8 9 10 11 12
Sample 25bp ladder V4S3A V4S3B V4S3C V4S3D V6S3A V6S3B V6S3C V6S3D
2. Run for 25 minutes at 250V

Gel Image

Lambda Exonuclease Digestion