Matt:LabNotes/2013-7-19: Difference between revisions

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### /home/kunzhang/softwares/bowtie2-latest/bowtie2 -k 1 --phred64 --mp 1,0 --rdg 0,1 --rfg 0,1 -x /home/mzcai/InSitu_HL155_130628_Analysis/Agi26kprobes_50bp -q /home/mzcai/InSitu_HL155_130628_Analysis/s_3_1_Indx12.txt > /home/mzcai/InSitu_HL155_130628_Analysis/Readsalign2probes_20gap50bp.txt &<br>mzcai@genome-miner:~/InSitu_HL155_130628_Analysis$ 17308686 reads; of these:<br>17308686 (100.00%) were unpaired; of these:<br>17112441 (98.87%) aligned 0 times<br>196245 (1.13%) aligned exactly 1 time<br>0 (0.00%) aligned >1 times<br>1.13% overall alignment rate<br>
### /home/kunzhang/softwares/bowtie2-latest/bowtie2 -k 1 --phred64 --mp 1,0 --rdg 0,1 --rfg 0,1 -x /home/mzcai/InSitu_HL155_130628_Analysis/Agi26kprobes_50bp -q /home/mzcai/InSitu_HL155_130628_Analysis/s_3_1_Indx12.txt > /home/mzcai/InSitu_HL155_130628_Analysis/Readsalign2probes_20gap50bp.txt &<br>mzcai@genome-miner:~/InSitu_HL155_130628_Analysis$ 17308686 reads; of these:<br>17308686 (100.00%) were unpaired; of these:<br>17112441 (98.87%) aligned 0 times<br>196245 (1.13%) aligned exactly 1 time<br>0 (0.00%) aligned >1 times<br>1.13% overall alignment rate<br>
# Counts for each probe: CountsofAgi26k_0gap50bp.txt, CountsofAgi26k_0gapFull.txt, CountsofAgi26k_20gap50bp.txt, CountsofAgi26k_20gapFull.txt
# Counts for each probe: CountsofAgi26k_0gap50bp.txt, CountsofAgi26k_0gapFull.txt, CountsofAgi26k_20gap50bp.txt, CountsofAgi26k_20gapFull.txt
## Counts aren't zero where expected...
## Many 0 gap reads aligned to 20 gap probes and vice versa suggests this method isn't specific enough
## Below are number of probes binned into number of reads
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''0 gap reads aligned to 0 gap probe'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''0 gap reads aligned to 20 gap probes'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''20 gap reads aligned to 0 gap probes'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''20 gap reads aligned to 20 gap probes'''
| align="center" style="background:#f0f0f0;"|''''''
|-
| Bin||Frequency||Bin||Frequency||Bin||Frequency||Bin||Frequency
|-
| 0||19||0||1317||0||4034||0||5
|-
| 7.157894737||293||19.10619469||11167||3.929824561||7384||9.902654867||4268
|-
| 14.31578947||1427||38.21238938||310||7.859649123||1185||19.80530973||7286
|-
| 21.47368421||3791||57.31858407||73||11.78947368||289||29.7079646||1191
|-
| 28.63157895||4282||76.42477876||31||15.71929825||135||39.61061947||111
|-
| 35.78947368||2293||95.53097345||20||19.64912281||57||49.51327434||37
|-
| 42.94736842||783||114.6371681||12||23.57894737||30||59.4159292||19
|-
| 50.10526316||186||133.7433628||8||27.50877193||20||69.31858407||13
|-
| 57.26315789||37||152.8495575||2||31.43859649||8||79.22123894||8
|-
| 64.42105263||12||171.9557522||3||35.36842105||4||89.12389381||4
|-
| 71.57894737||19||191.0619469||1||39.29824561||4||99.02654867||3
|-
| 78.73684211||8||210.1681416||3||43.22807018||6||108.9292035||0
|-
| 85.89473684||10||229.2743363||2||47.15789474||3||118.8318584||1
|-
| 93.05263158||6||248.380531||3||51.0877193||0||128.7345133||2
|-
| 100.2105263||4||267.4867257||1||55.01754386||0||138.6371681||2
|-
| 107.3684211||1||286.5929204||1||58.94736842||3||148.539823||0
|-
| 114.5263158||1||305.699115||0||62.87719298||1||158.4424779||5
|-
| 121.6842105||1||324.8053097||1||66.80701754||0||168.3451327||1
|-
| 128.8421053||0||343.9115044||1||70.73684211||3||178.2477876||0
|-
| 136||0||363.0176991||0||74.66666667||0||188.1504425||0
|-
| 143.1578947||0||382.1238938||1||78.59649123||0||198.0530973||0
|-
| 150.3157895||0||401.2300885||0||82.52631579||0||207.9557522||0
|-
| 157.4736842||0||420.3362832||0||86.45614035||0||217.8584071||1
|-
| 164.6315789||0||439.4424779||1||90.38596491||1||227.7610619||0
|-
| 171.7894737||1||458.5486726||0||94.31578947||0||237.6637168||0
|-
| 178.9473684||0||477.6548673||0||98.24561404||0||247.5663717||0
|-
| 186.1052632||1||496.7610619||0||102.1754386||0||257.4690265||1
|-
| 193.2631579||0||515.8672566||0||106.1052632||1||267.3716814||1
|-
| 200.4210526||0||534.9734513||0||110.0350877||1||277.2743363||0
|-
| 207.5789474||0||554.079646||0||113.9649123||0||287.1769912||0
|-
| 214.7368421||0||573.1858407||1||117.8947368||1||297.079646||0
|-
| 221.8947368||0||592.2920354||0||121.8245614||2||306.9823009||0
|-
| 229.0526316||0||611.3982301||0||125.754386||1||316.8849558||0
|-
| 236.2105263||0||630.5044248||0||129.6842105||0||326.7876106||0
|-
| 243.3684211||0||649.6106195||1||133.6140351||0||336.6902655||1
|-
| 250.5263158||0||668.7168142||0||137.5438596||0||346.5929204||0
|-
| 257.6842105||0||687.8230088||0||141.4736842||1||356.4955752||0
|-
| 264.8421053||1||706.9292035||0||145.4035088||0||366.3982301||0
|-
| 272||0||726.0353982||0||149.3333333||0||376.300885||0
|-
| 279.1578947||0||745.1415929||0||153.2631579||1||386.2035398||0
|-
| 286.3157895||0||764.2477876||0||157.1929825||2||396.1061947||0
|-
| 293.4736842||0||783.3539823||0||161.122807||0||406.0088496||0
|-
| 300.6315789||0||802.460177||0||165.0526316||0||415.9115044||0
|-
| 307.7894737||0||821.5663717||0||168.9824561||0||425.8141593||0
|-
| 314.9473684||0||840.6725664||0||172.9122807||0||435.7168142||0
|-
| 322.1052632||0||859.7787611||0||176.8421053||0||445.619469||0
|-
| 329.2631579||0||878.8849558||0||180.7719298||0||455.5221239||0
|-
| 336.4210526||0||897.9911504||0||184.7017544||1||465.4247788||0
|-
| 343.5789474||1||917.0973451||0||188.6315789||0||475.3274336||0
|-
| 350.7368421||0||936.2035398||0||192.5614035||0||485.2300885||0
|-
| 357.8947368||0||955.3097345||0||196.4912281||0||495.1327434||0
|-
| 365.0526316||0||974.4159292||0||200.4210526||0||505.0353982||0
|-
| 372.2105263||0||993.5221239||0||204.3508772||0||514.9380531||0
|-
| 379.3684211||0||1012.628319||0||208.2807018||0||524.840708||0
|-
| 386.5263158||0||1031.734513||0||212.2105263||0||534.7433628||0
|-
| 393.6842105||1||1050.840708||0||216.1403509||0||544.6460177||0
|-
| 400.8421053||0||1069.946903||0||220.0701754||0||554.5486726||0
|-
| 408||0||1089.053097||0||224||0||564.4513274||1
|-
| 415.1578947||0||1108.159292||0||227.9298246||0||574.3539823||0
|-
| 422.3157895||0||1127.265487||0||231.8596491||0||584.2566372||0
|-
| 429.4736842||0||1146.371681||1||235.7894737||0||594.159292||0
|-
| 436.6315789||0||1165.477876||0||239.7192982||0||604.0619469||0
|-
| 443.7894737||0||1184.584071||0||243.6491228||0||613.9646018||0
|-
| 450.9473684||0||1203.690265||0||247.5789474||0||623.8672566||0
|-
| 458.1052632||0||1222.79646||0||251.5087719||0||633.7699115||0
|-
| 465.2631579||0||1241.902655||0||255.4385965||0||643.6725664||0
|-
| 472.4210526||0||1261.00885||0||259.3684211||0||653.5752212||0
|-
| 479.5789474||0||1280.115044||0||263.2982456||0||663.4778761||0
|-
| 486.7368421||0||1299.221239||0||267.2280702||0||673.380531||0
|-
| 493.8947368||0||1318.327434||0||271.1578947||0||683.2831858||0
|-
| 501.0526316||0||1337.433628||0||275.0877193||0||693.1858407||0
|-
| 508.2105263||0||1356.539823||0||279.0175439||0||703.0884956||0
|-
| 515.3684211||0||1375.646018||0||282.9473684||0||712.9911504||0
|-
| 522.5263158||0||1394.752212||0||286.877193||0||722.8938053||0
|-
| 529.6842105||0||1413.858407||0||290.8070175||0||732.7964602||0
|-
| 536.8421053||0||1432.964602||0||294.7368421||0||742.699115||0
|-
| 544||0||1452.070796||0||298.6666667||0||752.6017699||0
|-
| 551.1578947||0||1471.176991||0||302.5964912||0||762.5044248||0
|-
| 558.3157895||0||1490.283186||0||306.5263158||0||772.4070796||0
|-
| 565.4736842||0||1509.389381||0||310.4561404||0||782.3097345||1
|-
| 572.6315789||0||1528.495575||0||314.3859649||0||792.2123894||0
|-
| 579.7894737||0||1547.60177||0||318.3157895||0||802.1150442||0
|-
| 586.9473684||0||1566.707965||0||322.245614||0||812.0176991||0
|-
| 594.1052632||0||1585.814159||0||326.1754386||0||821.920354||0
|-
| 601.2631579||0||1604.920354||0||330.1052632||0||831.8230088||0
|-
| 608.4210526||0||1624.026549||0||334.0350877||0||841.7256637||0
|-
| 615.5789474||0||1643.132743||0||337.9649123||0||851.6283186||0
|-
| 622.7368421||0||1662.238938||0||341.8947368||0||861.5309735||0
|-
| 629.8947368||0||1681.345133||0||345.8245614||0||871.4336283||0
|-
| 637.0526316||0||1700.451327||0||349.754386||0||881.3362832||0
|-
| 644.2105263||0||1719.557522||0||353.6842105||0||891.2389381||0
|-
| 651.3684211||0||1738.663717||0||357.6140351||0||901.1415929||0
|-
| 658.5263158||0||1757.769912||0||361.5438596||0||911.0442478||0
|-
| 665.6842105||0||1776.876106||0||365.4736842||0||920.9469027||0
|-
| 672.8421053||0||1795.982301||0||369.4035088||0||930.8495575||0
|-
| 680||0||1815.088496||0||373.3333333||0||940.7522124||0
|-
| 687.1578947||0||1834.19469||0||377.2631579||0||950.6548673||0
|-
| 694.3157895||0||1853.300885||1||381.1929825||0||960.5575221||0
|-
| 701.4736842||0||1872.40708||0||385.122807||0||970.460177||0
|-
| 708.6315789||0||1891.513274||0||389.0526316||0||980.3628319||0
|-
| 715.7894737||0||1910.619469||0||392.9824561||0||990.2654867||0
|-
| 722.9473684||0||1929.725664||0||396.9122807||0||1000.168142||0
|-
| 730.1052632||0||1948.831858||0||400.8421053||0||1010.070796||0
|-
| 737.2631579||0||1967.938053||0||404.7719298||0||1019.973451||0
|-
| 744.4210526||0||1987.044248||0||408.7017544||0||1029.876106||0
|-
| 751.5789474||0||2006.150442||0||412.6315789||0||1039.778761||0
|-
| 758.7368421||0||2025.256637||0||416.5614035||0||1049.681416||0
|-
| 765.8947368||0||2044.362832||0||420.4912281||0||1059.584071||0
|-
| 773.0526316||0||2063.469027||0||424.4210526||0||1069.486726||0
|-
| 780.2105263||0||2082.575221||0||428.3508772||0||1079.389381||0
|-
| 787.3684211||0||2101.681416||0||432.2807018||0||1089.292035||0
|-
| 794.5263158||0||2120.787611||0||436.2105263||0||1099.19469||0
|-
| 801.6842105||0||2139.893805||0||440.1403509||0||1109.097345||0
|-
| 808.8421053||0||More||1||444.0701754||0||More||1
|-
| More||1||||||More||1||||
|}

Revision as of 21:54, 22 July 2013

Analysis of HL155: Representation Bias of Agi26k Oligos

  • Previously amplified Agi26k oligos with sequence adapters to sequence
  • By counting the number of reads for each probe, can determine uniformity of oligos in probe set
  1. Turn FISSEQ_Probes_Dec2012.txt into fasta file (Full: Agi26kprobes_to_order.fa 50bp: Agi26kprobes_to_order_50bp.fa)
    1. First Primer for V6: "GTCATATCGGTCACTGTT"
    2. First Primer for V4: "TAGACTGGAAGAGCACTGTT"
  2. Build index for probes
    1. /home/kunzhang/softwares/bowtie2-latest/bowtie2-build ~/InSitu_HL155_130628_Analysis/Agi26kprobes_to_order.fa Agi26kprobes
    2. /home/kunzhang/softwares/bowtie2-latest/bowtie2-build ~/InSitu_HL155_130628_Analysis/Agi26kprobes_to_order_50bp.fa Agi26kprobes_50bp
  3. Align to index
    1. Indx10 -> 0 gap -> V6:
      1. /home/kunzhang/softwares/bowtie2-latest/bowtie2 -k 1 --phred64 --mp 1,0 --rdg 0,1 --rfg 0,1 -x /home/mzcai/InSitu_HL155_130628_Analysis/Agi26kprobes -q /home/mzcai/InSitu_HL155_130628_Analysis/s_3_1_Indx10.txt > /home/mzcai/InSitu_HL155_130628_Analysis/Readsalign2probes_0gapFull.txt &
        16368416 reads; of these:
        16368416 (100.00%) were unpaired; of these:
        8915822 (54.47%) aligned 0 times
        7452594 (45.53%) aligned exactly 1 time
        0 (0.00%) aligned >1 times
        45.53% overall alignment rate
      2. /home/kunzhang/softwares/bowtie2-latest/bowtie2 -k 1 --phred64 --mp 1,0 --rdg 0,1 --rfg 0,1 -x /home/mzcai/InSitu_HL155_130628_Analysis/Agi26kprobes_50bp -q /home/mzcai/InSitu_HL155_130628_Analysis/s_3_1_Indx10.txt > /home/mzcai/InSitu_HL155_130628_Analysis/Readsalign2probes_0gap50bp.txt &
        mzcai@genome-miner:~/InSitu_HL155_130628_Analysis$ 16368416 reads; of these:
        16368416 (100.00%) were unpaired; of these:
        15978678 (97.62%) aligned 0 times
        389738 (2.38%) aligned exactly 1 time
        0 (0.00%) aligned >1 times
        2.38% overall alignment rate
    2. Indx12 -> 20 gap -> V4:
      1. /home/kunzhang/softwares/bowtie2-latest/bowtie2 -k 1 --phred64 --mp 1,0 --rdg 0,1 --rfg 0,1 -x /home/mzcai/InSitu_HL155_130628_Analysis/Agi26kprobes -q /home/mzcai/InSitu_HL155_130628_Analysis/s_3_1_Indx12.txt > /home/mzcai/InSitu_HL155_130628_Analysis/Readsalign2probes_20gapFull.txt &
        mzcai@genome-miner:~/InSitu_HL155_130628_Analysis$ 17308686 reads; of these:
        17308686 (100.00%) were unpaired; of these:
        11482192 (66.34%) aligned 0 times
        5826494 (33.66%) aligned exactly 1 time
        0 (0.00%) aligned >1 times
        33.66% overall alignment rate
      2. /home/kunzhang/softwares/bowtie2-latest/bowtie2 -k 1 --phred64 --mp 1,0 --rdg 0,1 --rfg 0,1 -x /home/mzcai/InSitu_HL155_130628_Analysis/Agi26kprobes_50bp -q /home/mzcai/InSitu_HL155_130628_Analysis/s_3_1_Indx12.txt > /home/mzcai/InSitu_HL155_130628_Analysis/Readsalign2probes_20gap50bp.txt &
        mzcai@genome-miner:~/InSitu_HL155_130628_Analysis$ 17308686 reads; of these:
        17308686 (100.00%) were unpaired; of these:
        17112441 (98.87%) aligned 0 times
        196245 (1.13%) aligned exactly 1 time
        0 (0.00%) aligned >1 times
        1.13% overall alignment rate
  4. Counts for each probe: CountsofAgi26k_0gap50bp.txt, CountsofAgi26k_0gapFull.txt, CountsofAgi26k_20gap50bp.txt, CountsofAgi26k_20gapFull.txt
    1. Many 0 gap reads aligned to 20 gap probes and vice versa suggests this method isn't specific enough
    2. Below are number of probes binned into number of reads
0 gap reads aligned to 0 gap probe ' 0 gap reads aligned to 20 gap probes ' 20 gap reads aligned to 0 gap probes ' 20 gap reads aligned to 20 gap probes '
Bin Frequency Bin Frequency Bin Frequency Bin Frequency
0 19 0 1317 0 4034 0 5
7.157894737 293 19.10619469 11167 3.929824561 7384 9.902654867 4268
14.31578947 1427 38.21238938 310 7.859649123 1185 19.80530973 7286
21.47368421 3791 57.31858407 73 11.78947368 289 29.7079646 1191
28.63157895 4282 76.42477876 31 15.71929825 135 39.61061947 111
35.78947368 2293 95.53097345 20 19.64912281 57 49.51327434 37
42.94736842 783 114.6371681 12 23.57894737 30 59.4159292 19
50.10526316 186 133.7433628 8 27.50877193 20 69.31858407 13
57.26315789 37 152.8495575 2 31.43859649 8 79.22123894 8
64.42105263 12 171.9557522 3 35.36842105 4 89.12389381 4
71.57894737 19 191.0619469 1 39.29824561 4 99.02654867 3
78.73684211 8 210.1681416 3 43.22807018 6 108.9292035 0
85.89473684 10 229.2743363 2 47.15789474 3 118.8318584 1
93.05263158 6 248.380531 3 51.0877193 0 128.7345133 2
100.2105263 4 267.4867257 1 55.01754386 0 138.6371681 2
107.3684211 1 286.5929204 1 58.94736842 3 148.539823 0
114.5263158 1 305.699115 0 62.87719298 1 158.4424779 5
121.6842105 1 324.8053097 1 66.80701754 0 168.3451327 1
128.8421053 0 343.9115044 1 70.73684211 3 178.2477876 0
136 0 363.0176991 0 74.66666667 0 188.1504425 0
143.1578947 0 382.1238938 1 78.59649123 0 198.0530973 0
150.3157895 0 401.2300885 0 82.52631579 0 207.9557522 0
157.4736842 0 420.3362832 0 86.45614035 0 217.8584071 1
164.6315789 0 439.4424779 1 90.38596491 1 227.7610619 0
171.7894737 1 458.5486726 0 94.31578947 0 237.6637168 0
178.9473684 0 477.6548673 0 98.24561404 0 247.5663717 0
186.1052632 1 496.7610619 0 102.1754386 0 257.4690265 1
193.2631579 0 515.8672566 0 106.1052632 1 267.3716814 1
200.4210526 0 534.9734513 0 110.0350877 1 277.2743363 0
207.5789474 0 554.079646 0 113.9649123 0 287.1769912 0
214.7368421 0 573.1858407 1 117.8947368 1 297.079646 0
221.8947368 0 592.2920354 0 121.8245614 2 306.9823009 0
229.0526316 0 611.3982301 0 125.754386 1 316.8849558 0
236.2105263 0 630.5044248 0 129.6842105 0 326.7876106 0
243.3684211 0 649.6106195 1 133.6140351 0 336.6902655 1
250.5263158 0 668.7168142 0 137.5438596 0 346.5929204 0
257.6842105 0 687.8230088 0 141.4736842 1 356.4955752 0
264.8421053 1 706.9292035 0 145.4035088 0 366.3982301 0
272 0 726.0353982 0 149.3333333 0 376.300885 0
279.1578947 0 745.1415929 0 153.2631579 1 386.2035398 0
286.3157895 0 764.2477876 0 157.1929825 2 396.1061947 0
293.4736842 0 783.3539823 0 161.122807 0 406.0088496 0
300.6315789 0 802.460177 0 165.0526316 0 415.9115044 0
307.7894737 0 821.5663717 0 168.9824561 0 425.8141593 0
314.9473684 0 840.6725664 0 172.9122807 0 435.7168142 0
322.1052632 0 859.7787611 0 176.8421053 0 445.619469 0
329.2631579 0 878.8849558 0 180.7719298 0 455.5221239 0
336.4210526 0 897.9911504 0 184.7017544 1 465.4247788 0
343.5789474 1 917.0973451 0 188.6315789 0 475.3274336 0
350.7368421 0 936.2035398 0 192.5614035 0 485.2300885 0
357.8947368 0 955.3097345 0 196.4912281 0 495.1327434 0
365.0526316 0 974.4159292 0 200.4210526 0 505.0353982 0
372.2105263 0 993.5221239 0 204.3508772 0 514.9380531 0
379.3684211 0 1012.628319 0 208.2807018 0 524.840708 0
386.5263158 0 1031.734513 0 212.2105263 0 534.7433628 0
393.6842105 1 1050.840708 0 216.1403509 0 544.6460177 0
400.8421053 0 1069.946903 0 220.0701754 0 554.5486726 0
408 0 1089.053097 0 224 0 564.4513274 1
415.1578947 0 1108.159292 0 227.9298246 0 574.3539823 0
422.3157895 0 1127.265487 0 231.8596491 0 584.2566372 0
429.4736842 0 1146.371681 1 235.7894737 0 594.159292 0
436.6315789 0 1165.477876 0 239.7192982 0 604.0619469 0
443.7894737 0 1184.584071 0 243.6491228 0 613.9646018 0
450.9473684 0 1203.690265 0 247.5789474 0 623.8672566 0
458.1052632 0 1222.79646 0 251.5087719 0 633.7699115 0
465.2631579 0 1241.902655 0 255.4385965 0 643.6725664 0
472.4210526 0 1261.00885 0 259.3684211 0 653.5752212 0
479.5789474 0 1280.115044 0 263.2982456 0 663.4778761 0
486.7368421 0 1299.221239 0 267.2280702 0 673.380531 0
493.8947368 0 1318.327434 0 271.1578947 0 683.2831858 0
501.0526316 0 1337.433628 0 275.0877193 0 693.1858407 0
508.2105263 0 1356.539823 0 279.0175439 0 703.0884956 0
515.3684211 0 1375.646018 0 282.9473684 0 712.9911504 0
522.5263158 0 1394.752212 0 286.877193 0 722.8938053 0
529.6842105 0 1413.858407 0 290.8070175 0 732.7964602 0
536.8421053 0 1432.964602 0 294.7368421 0 742.699115 0
544 0 1452.070796 0 298.6666667 0 752.6017699 0
551.1578947 0 1471.176991 0 302.5964912 0 762.5044248 0
558.3157895 0 1490.283186 0 306.5263158 0 772.4070796 0
565.4736842 0 1509.389381 0 310.4561404 0 782.3097345 1
572.6315789 0 1528.495575 0 314.3859649 0 792.2123894 0
579.7894737 0 1547.60177 0 318.3157895 0 802.1150442 0
586.9473684 0 1566.707965 0 322.245614 0 812.0176991 0
594.1052632 0 1585.814159 0 326.1754386 0 821.920354 0
601.2631579 0 1604.920354 0 330.1052632 0 831.8230088 0
608.4210526 0 1624.026549 0 334.0350877 0 841.7256637 0
615.5789474 0 1643.132743 0 337.9649123 0 851.6283186 0
622.7368421 0 1662.238938 0 341.8947368 0 861.5309735 0
629.8947368 0 1681.345133 0 345.8245614 0 871.4336283 0
637.0526316 0 1700.451327 0 349.754386 0 881.3362832 0
644.2105263 0 1719.557522 0 353.6842105 0 891.2389381 0
651.3684211 0 1738.663717 0 357.6140351 0 901.1415929 0
658.5263158 0 1757.769912 0 361.5438596 0 911.0442478 0
665.6842105 0 1776.876106 0 365.4736842 0 920.9469027 0
672.8421053 0 1795.982301 0 369.4035088 0 930.8495575 0
680 0 1815.088496 0 373.3333333 0 940.7522124 0
687.1578947 0 1834.19469 0 377.2631579 0 950.6548673 0
694.3157895 0 1853.300885 1 381.1929825 0 960.5575221 0
701.4736842 0 1872.40708 0 385.122807 0 970.460177 0
708.6315789 0 1891.513274 0 389.0526316 0 980.3628319 0
715.7894737 0 1910.619469 0 392.9824561 0 990.2654867 0
722.9473684 0 1929.725664 0 396.9122807 0 1000.168142 0
730.1052632 0 1948.831858 0 400.8421053 0 1010.070796 0
737.2631579 0 1967.938053 0 404.7719298 0 1019.973451 0
744.4210526 0 1987.044248 0 408.7017544 0 1029.876106 0
751.5789474 0 2006.150442 0 412.6315789 0 1039.778761 0
758.7368421 0 2025.256637 0 416.5614035 0 1049.681416 0
765.8947368 0 2044.362832 0 420.4912281 0 1059.584071 0
773.0526316 0 2063.469027 0 424.4210526 0 1069.486726 0
780.2105263 0 2082.575221 0 428.3508772 0 1079.389381 0
787.3684211 0 2101.681416 0 432.2807018 0 1089.292035 0
794.5263158 0 2120.787611 0 436.2105263 0 1099.19469 0
801.6842105 0 2139.893805 0 440.1403509 0 1109.097345 0
808.8421053 0 More 1 444.0701754 0 More 1
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