Hosuk:LabNotesMDA/2013-7-18: Difference between revisions

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(Created page with "*LabNote ===MDA with PGP1F, 8th, Dev v9.1=== *Use new device (v9.1) ====KOH concentration for (ALS+Cell) mix==== *I...")
 
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====KOH concentration for (ALS+Cell) mix====
====KOH concentration for (ALS+Cell) mix====
*In 20uL reaction, the final concentration of KOH in ALS while denaturing is '''240mM'''.
*In 20uL reaction, the final concentration of KOH in ALS while denaturing is '''240mM'''.
*In the prev version,269mM of KOH in ALS buffer has used ([[Hosuk:LabNotesMDA/2013-7-2|v7.3 ring mixer device]]).
*In the v7.3 version, '''269mM''' of KOH in ALS buffer has used ([[Hosuk:LabNotesMDA/2013-7-2|v7.3 ring mixer device]]).
*For this v9.1 device, 286mM of KOH in ALS buffer is used (Cell : (ALS-Cell) = 1 : 5.247).
*For this v9.1 device, '''286mM''' of KOH in ALS buffer is used (Cell : (ALS-Cell) = 1 : 5.247).




*[[File:ALS_Recipe.png|600px]]
*[[File:ALS_Recipe.png|600px]]

Revision as of 18:30, 26 July 2013


MDA with PGP1F, 8th, Dev v9.1

  • Use new device (v9.1)


KOH concentration for (ALS+Cell) mix

  • In 20uL reaction, the final concentration of KOH in ALS while denaturing is 240mM.
  • In the v7.3 version, 269mM of KOH in ALS buffer has used (v7.3 ring mixer device).
  • For this v9.1 device, 286mM of KOH in ALS buffer is used (Cell : (ALS-Cell) = 1 : 5.247).