Hosuk:LabNotesMDA/2013-7-18: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===MDA with PGP1F, 8th, Dev v9.1=== *Use new device (v9.1) ====KOH concentration for (ALS+Cell) mix==== *I...") |
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====KOH concentration for (ALS+Cell) mix==== | ====KOH concentration for (ALS+Cell) mix==== | ||
*In 20uL reaction, the final concentration of KOH in ALS while denaturing is '''240mM'''. | *In 20uL reaction, the final concentration of KOH in ALS while denaturing is '''240mM'''. | ||
*In the | *In the v7.3 version, '''269mM''' of KOH in ALS buffer has used ([[Hosuk:LabNotesMDA/2013-7-2|v7.3 ring mixer device]]). | ||
*For this v9.1 device, 286mM of KOH in ALS buffer is used (Cell : (ALS-Cell) = 1 : 5.247). | *For this v9.1 device, '''286mM''' of KOH in ALS buffer is used (Cell : (ALS-Cell) = 1 : 5.247). | ||
*[[File:ALS_Recipe.png|600px]] | *[[File:ALS_Recipe.png|600px]] |
Revision as of 18:30, 26 July 2013
MDA with PGP1F, 8th, Dev v9.1
- Use new device (v9.1)
KOH concentration for (ALS+Cell) mix
- In 20uL reaction, the final concentration of KOH in ALS while denaturing is 240mM.
- In the v7.3 version, 269mM of KOH in ALS buffer has used (v7.3 ring mixer device).
- For this v9.1 device, 286mM of KOH in ALS buffer is used (Cell : (ALS-Cell) = 1 : 5.247).