Hosuk:LabNotesMDA/2013-7-26: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===MDA with PGP1F, 11th, Dev v9.1=== *Use new device (v9.1) *Same experiment to [[Hosuk:LabNotesMDA/2013-7-1...") |
>Hosuki78 (→Result) |
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*[[File:qPCR_Result_MDAv9.1_2013-07-26_fig1_QGraph_Table.png| | *[[File:qPCR_Result_MDAv9.1_2013-07-26_fig1_QGraph_Table.png|650px]] | ||
*[[File:qPCR_Result_MDAv9.1_2013-07-26_fig2_History.png| | *[[File:qPCR_Result_MDAv9.1_2013-07-26_fig2_History.png|550px]] |
Revision as of 19:00, 26 July 2013
MDA with PGP1F, 11th, Dev v9.1
- Use new device (v9.1)
- Same experiment to 07/18, but try pushing ALS+Cell into compartments with H2O, not air.
Experiment
- Start : 5:30pm 07/25, End : 8:30am 07/26 --> about 15hr reaction
- qPCR run for verification
- Extract just about 1.1 uL at each tube and run qPCR.
Result
- Good, considering bad experiment condition
- First(Left) two chambers had lower amplicons because of less template, but it’s better than pushing with air.
- Total amount of DNA estimated were lower than previous run (07/18),
- but the distribution of amount of DNAs was relatively even though no active mixing!
- It looked enough amount for tagmentation