Daniel:Notebook/HiResChrPaint/2013-8-1: Difference between revisions
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>Djacobse (Created page with "==Troubleshooting qPCR (Started 7/31/2013)== Back to Calendar ===PCR 2 (Me)...") |
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==Troubleshooting qPCR ([[Daniel:Notebook/HiResChrPaint/2013-7-31#Troubleshooting qPCR|Started 7/31/2013]])== | ==Troubleshooting qPCR ([[Daniel:Notebook/HiResChrPaint/2013-7-31#Troubleshooting qPCR|Started 7/31/2013]])== | ||
[[ | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | ||
===PCR 2 (Me)=== | ===PCR 2 (Me)=== | ||
As per yesterday's finale, I'm trying single reaction PCR again, comparing Noi and my Master Mixes, and using the Eppendorf thermocycler instead of the BioRad one. | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | ||
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===qPCR Results=== |
Revision as of 21:43, 1 August 2013
Troubleshooting qPCR (Started 7/31/2013)
PCR 2 (Me)
As per yesterday's finale, I'm trying single reaction PCR again, comparing Noi and my Master Mixes, and using the Eppendorf thermocycler instead of the BioRad one.
20 nM OligoPool | 2X Kapa SYBR qPCR MM | 100 uM AP1V41U | 100 uM AP2V4 | 100 uM AP1V61U | 100 uM AP2V6 | 2 mM aa-dUTP | H20 | Total Volume | |
V4S1D | 0.5 | 50 | 0.5 | 0.5 | 0.5 | 0.5 | 0 | 47.5 | 100 |
V4S1N | 0.5 | 50 | 0.5 | 0.5 | 0.5 | 0.5 | 0 | 47.5 | 100 |