Daniel:Notebook/HiResChrPaint/2013-8-1: Difference between revisions

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==Troubleshooting qPCR ([[Daniel:Notebook/HiResChrPaint/2013-7-31#Troubleshooting qPCR|Started 7/31/2013]])==
==Troubleshooting qPCR ([[Daniel:Notebook/HiResChrPaint/2013-7-31#Troubleshooting qPCR|Started 7/31/2013]])==


[[Danie:Notebook/HiResChrPaint|Back to Calendar]]
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]


===PCR 2 (Me)===
===PCR 2 (Me)===


Since I'm pretty sure that the PCR is working fine, except that the master mix I used wasn't working, I'm going to try again (2 reactions only!) and see if I can't PCR something, one using my MM and one using Noi's.
As per yesterday's finale, I'm trying single reaction PCR again, comparing Noi and my Master Mixes, and using the Eppendorf thermocycler instead of the BioRad one.


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===qPCR Results===

Revision as of 21:43, 1 August 2013

Troubleshooting qPCR (Started 7/31/2013)

Back to Calendar

PCR 2 (Me)

As per yesterday's finale, I'm trying single reaction PCR again, comparing Noi and my Master Mixes, and using the Eppendorf thermocycler instead of the BioRad one.

  20 nM OligoPool 2X Kapa SYBR qPCR MM 100 uM AP1V41U 100 uM AP2V4 100 uM AP1V61U 100 uM AP2V6 2 mM aa-dUTP H20 Total Volume
V4S1D 0.5 50 0.5 0.5 0.5 0.5 0 47.5 100
V4S1N 0.5 50 0.5 0.5 0.5 0.5 0 47.5 100

qPCR Results