Daniel:Notebook/HiResChrPaint/2013-8-1: Difference between revisions
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== | =FISH= | ||
Hosuk and I went to the microscope room today. We got a few images that might be worthwhile. We also looked closer at the microscopes used by Beliveau et al, and we realized that two of the microscopes they mention are almost exactly the same models we use. One matches the confocal in the basement, the other matches the Olympus in 406. So I can and should use the Olympus from now on to screen for good results. | |||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | ||
==Troubleshooting qPCR ([[Daniel:Notebook/HiResChrPaint/2013-7-31#Troubleshooting qPCR|Started 7/31/2013]])== | |||
===PCR 2 (Me)=== | ===PCR 2 (Me)=== |
Revision as of 21:45, 1 August 2013
FISH
Hosuk and I went to the microscope room today. We got a few images that might be worthwhile. We also looked closer at the microscopes used by Beliveau et al, and we realized that two of the microscopes they mention are almost exactly the same models we use. One matches the confocal in the basement, the other matches the Olympus in 406. So I can and should use the Olympus from now on to screen for good results.
Troubleshooting qPCR (Started 7/31/2013)
PCR 2 (Me)
As per yesterday's finale, I'm trying single reaction PCR again, comparing Noi and my Master Mixes, and using the Eppendorf thermocycler instead of the BioRad one.
20 nM OligoPool | 2X Kapa SYBR qPCR MM | 100 uM AP1V41U | 100 uM AP2V4 | 100 uM AP1V61U | 100 uM AP2V6 | 2 mM aa-dUTP | H20 | Total Volume | |
V4S1D | 0.5 | 50 | 0.5 | 0.5 | 0.5 | 0.5 | 0 | 47.5 | 100 |
V4S1N | 0.5 | 50 | 0.5 | 0.5 | 0.5 | 0.5 | 0 | 47.5 | 100 |