Daniel:Notebook/HiResChrPaint/2013-8-10: Difference between revisions
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>Djacobse (Created page with " ==Dye Coupling== *Following ULYSIS Protocol 1. Resuspend DNA in 20 uL labeling buffer (Component C) 2. Denature DNA for 5 minu...") |
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=S2 Probe Prep (ULS; [[Daniel:Notebook/HiResChrPaint/2013-8-8|Started 8/8/2013]])= | |||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | |||
==Dye Coupling== | ==Dye Coupling== | ||
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1. Resuspend DNA in 20 uL labeling buffer (Component C) | 1. Resuspend DNA in 20 uL labeling buffer (Component C) | ||
2. Denature DNA for 5 minutes at 95C; snap cool on ice | 2. Denature DNA for 5 minutes at 95C; snap cool on ice | ||
3. Add 1 uL dye to sample | 3. Add 1 uL dye to sample per ug | ||
4. Incubate for 20 minutes at 80C, snap cool on ice | 4. Incubate for 20 minutes at 80C, snap cool on ice | ||
5. Purify using [[Daniel:Protocols/CentriSep|Centri Sep]] column | 5. Purify using [[Daniel:Protocols/CentriSep|Centri Sep]] column |
Revision as of 00:10, 11 August 2013
S2 Probe Prep (ULS; Started 8/8/2013)
Dye Coupling
- Following ULYSIS Protocol
1. Resuspend DNA in 20 uL labeling buffer (Component C) 2. Denature DNA for 5 minutes at 95C; snap cool on ice 3. Add 1 uL dye to sample per ug 4. Incubate for 20 minutes at 80C, snap cool on ice 5. Purify using Centri Sep column