Daniel:Notebook/HiResChrPaint/2013-8-10: Difference between revisions

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(Created page with " ==Dye Coupling== *Following ULYSIS Protocol 1. Resuspend DNA in 20 uL labeling buffer (Component C) 2. Denature DNA for 5 minu...")
 
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=S2 Probe Prep (ULS; [[Daniel:Notebook/HiResChrPaint/2013-8-8|Started 8/8/2013]])=


[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]


==Dye Coupling==
==Dye Coupling==
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  1. Resuspend DNA in 20 uL labeling buffer (Component C)
  1. Resuspend DNA in 20 uL labeling buffer (Component C)
  2. Denature DNA for 5 minutes at 95C; snap cool on ice
  2. Denature DNA for 5 minutes at 95C; snap cool on ice
  3. Add 1 uL dye to sample
  3. Add 1 uL dye to sample per ug
  4. Incubate for 20 minutes at 80C, snap cool on ice
  4. Incubate for 20 minutes at 80C, snap cool on ice
  5. Purify using [[Daniel:Protocols/CentriSep|Centri Sep]] column
  5. Purify using [[Daniel:Protocols/CentriSep|Centri Sep]] column

Revision as of 00:10, 11 August 2013

S2 Probe Prep (ULS; Started 8/8/2013)

Back to Calendar

Dye Coupling

1. Resuspend DNA in 20 uL labeling buffer (Component C)
2. Denature DNA for 5 minutes at 95C; snap cool on ice
3. Add 1 uL dye to sample per ug
4. Incubate for 20 minutes at 80C, snap cool on ice
5. Purify using Centri Sep column