Jeff:LabNotes/Midas Animation: Difference between revisions
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>Dinh mNo edit summary |
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*At 1:30 for the MDA, shouldn't the polymerases keep going and not get stuck? | *At 1:30 for the MDA, shouldn't the polymerases keep going and not get stuck? | ||
*At 2:24, why are the first 2 PCR tubes next to each other and all the others are organized every-other. It makes the first corner tube seem unique | *At 2:24, why are the first 2 PCR tubes next to each other and all the others are organized every-other. It makes the first corner tube seem unique | ||
Dinh's comments: <br> | |||
* Very cool how the color of the solution changes to indicate new mixtures. | |||
* I agree with Matt about the weird pronunciation for polymerase. | |||
* Does not mention how the fluorescence get incorporated with new DNA. | |||
* In first round the N3 is used then N6 is used for second round, and primer binding seems non-random. |
Revision as of 23:57, 22 August 2013
Matt's comments:
Really cool video and easy to understand! and all my comments here are just trying to be nit-picky
- At 00:45 should the DNA be fragmented? If so, should the voice mention that?
- Starting at 1:00 every time it says polymerase it sounds like "pall-mer-aze"
- At 1:30 for the MDA, shouldn't the polymerases keep going and not get stuck?
- At 2:24, why are the first 2 PCR tubes next to each other and all the others are organized every-other. It makes the first corner tube seem unique
Dinh's comments:
- Very cool how the color of the solution changes to indicate new mixtures.
- I agree with Matt about the weird pronunciation for polymerase.
- Does not mention how the fluorescence get incorporated with new DNA.
- In first round the N3 is used then N6 is used for second round, and primer binding seems non-random.