Jeff:LabNotes/Midas Animation: Difference between revisions
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*At 1:30 for the MDA, shouldn't the polymerases keep going and not get stuck? | *At 1:30 for the MDA, shouldn't the polymerases keep going and not get stuck? | ||
*At 2:24, why are the first 2 PCR tubes next to each other and all the others are organized every-other. It makes the first corner tube seem unique | *At 2:24, why are the first 2 PCR tubes next to each other and all the others are organized every-other. It makes the first corner tube seem unique | ||
Dinh's comments: <br> | |||
* Very cool how the color of the solution changes to indicate new mixtures. | |||
* I agree with Matt about the weird pronunciation for polymerase. | |||
* Does not mention how the fluorescence get incorporated with new DNA. | |||
* Primer binding pattern seems non-random, although the primer sequences are different, should it be evenly spaced? | |||
Noi's comments: <br> | |||
* This is cool Jeff!! | |||
* I am not sure that you assume that the viewers are already known about what is this animation about based on the title of the animation. I just feel like there should be a key word or short sentence to represent what is this animation about before starting with "the process begin with the..." | |||
* The display of microwell is very nice and clear about the details. | |||
* During MDA, I think you may already notice about the length of primers in the 1st and 2nd round of amplification are different | |||
* During DNA synthesis, I think it would be great to see how dNTP and fluorescent marker are incorporated to the new DNA strand (at least there should be molecule of fluorophores in dsDNA. | |||
* Is it too much details to make the 4 different colors of 4 bases? | |||
* Not sure if I am right. After binding of 2nd primer to the new strand DNA, did DNA synthesis go to the wrong direction (3'->5')? |
Latest revision as of 23:20, 26 August 2013
Matt's comments:
Really cool video and easy to understand! and all my comments here are just trying to be nit-picky
- At 00:45 should the DNA be fragmented? If so, should the voice mention that?
- Starting at 1:00 every time it says polymerase it sounds like "pall-mer-aze"
- At 1:30 for the MDA, shouldn't the polymerases keep going and not get stuck?
- At 2:24, why are the first 2 PCR tubes next to each other and all the others are organized every-other. It makes the first corner tube seem unique
Dinh's comments:
- Very cool how the color of the solution changes to indicate new mixtures.
- I agree with Matt about the weird pronunciation for polymerase.
- Does not mention how the fluorescence get incorporated with new DNA.
- Primer binding pattern seems non-random, although the primer sequences are different, should it be evenly spaced?
Noi's comments:
- This is cool Jeff!!
- I am not sure that you assume that the viewers are already known about what is this animation about based on the title of the animation. I just feel like there should be a key word or short sentence to represent what is this animation about before starting with "the process begin with the..."
- The display of microwell is very nice and clear about the details.
- During MDA, I think you may already notice about the length of primers in the 1st and 2nd round of amplification are different
- During DNA synthesis, I think it would be great to see how dNTP and fluorescent marker are incorporated to the new DNA strand (at least there should be molecule of fluorophores in dsDNA.
- Is it too much details to make the 4 different colors of 4 bases?
- Not sure if I am right. After binding of 2nd primer to the new strand DNA, did DNA synthesis go to the wrong direction (3'->5')?