Daniel:Notebook/HiResChrPaint/2013-9-11: Difference between revisions

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>Djacobse
(Created page with "=Fibroblast Adherence Timecourse (Started 09/09/2013)= Back to Calendar Petri dishes are in! So...")
 
>Djacobse
 
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'''New Template for Experiment'''
'''New Template for Experiment'''
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
| width="94" height="30" | Name
| width="65" | Surface
| width="65" | Trypsin Dilution
| width="76" | Cell Seeding Density
| width="88" | Media Kicker
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | Slide 09/09/13
| Slide
| 1:10
| align="center" | 1.00E+06
| None
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | Slide 1
| Slide
| 1:20
| align="center" | 1.00E+06
| None
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | Slide 2
| Slide
| 1:20
| align="center" | 1.00E+06
| 100 uL DMEM
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | Dish 1
| Dish
| 1:20
| align="center" | 1.00E+06
| None
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | Dish 2
| Dish
| 1:20
| align="center" | 1.00E+06
| 100 uL DMEM
|}
==Timecourse Experiment 09/11/2013==
1. Cultured cells from [[Daniel:Notebook/HiResChrPaint/2013-9-9|9/9/13 (step 6)]] (PGP1F, Passage 12)
2. Aspirate media and rinse with 5 mL PBS
3. Aspirate PBS and add 1 mL trypsin solution; incubate for several minutes
4. Add 9 mL DMEM to cell culture, mix by gently pipetting
5. Add 100 uL cell suspension to center of surface (2x slides, 2x dishes)
6. Took time course photos of slides at 3 hours and 6 hours
7. Continued [[Daniel:Notebook/HiResChrPaint/2013-9-12|tomorrow]]

Latest revision as of 23:51, 11 September 2013

Fibroblast Adherence Timecourse (Started 09/09/2013)[edit]

Back to Calendar

Petri dishes are in! So I've got a revised itinerary. First, a recap. There are a few important variables to track in this experiment:

  • (1) surface type (slide or dish)
  • (2) trypsin dilution (to ensure cells may attach)
  • (3) Cell seeding density (1,000,000 cells/mL recommended)
  • (4) Adding more media to help growth

New Template for Experiment

Name Surface Trypsin Dilution Cell Seeding Density Media Kicker
Slide 09/09/13 Slide 1:10 1.00E+06 None
Slide 1 Slide 1:20 1.00E+06 None
Slide 2 Slide 1:20 1.00E+06 100 uL DMEM
Dish 1 Dish 1:20 1.00E+06 None
Dish 2 Dish 1:20 1.00E+06 100 uL DMEM

Timecourse Experiment 09/11/2013[edit]

1. Cultured cells from 9/9/13 (step 6) (PGP1F, Passage 12)
2. Aspirate media and rinse with 5 mL PBS
3. Aspirate PBS and add 1 mL trypsin solution; incubate for several minutes
4. Add 9 mL DMEM to cell culture, mix by gently pipetting
5. Add 100 uL cell suspension to center of surface (2x slides, 2x dishes)
6. Took time course photos of slides at 3 hours and 6 hours
7. Continued tomorrow