Daniel:Notebook/HiResChrPaint/2013-9-11: Difference between revisions
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>Djacobse (Created page with "=Fibroblast Adherence Timecourse (Started 09/09/2013)= Back to Calendar Petri dishes are in! So...") |
>Djacobse |
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'''New Template for Experiment''' | '''New Template for Experiment''' | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt;font-weight:bold" align="center" | |||
| width="94" height="30" | Name | |||
| width="65" | Surface | |||
| width="65" | Trypsin Dilution | |||
| width="76" | Cell Seeding Density | |||
| width="88" | Media Kicker | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | Slide 09/09/13 | |||
| Slide | |||
| 1:10 | |||
| align="center" | 1.00E+06 | |||
| None | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | Slide 1 | |||
| Slide | |||
| 1:20 | |||
| align="center" | 1.00E+06 | |||
| None | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | Slide 2 | |||
| Slide | |||
| 1:20 | |||
| align="center" | 1.00E+06 | |||
| 100 uL DMEM | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | Dish 1 | |||
| Dish | |||
| 1:20 | |||
| align="center" | 1.00E+06 | |||
| None | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | Dish 2 | |||
| Dish | |||
| 1:20 | |||
| align="center" | 1.00E+06 | |||
| 100 uL DMEM | |||
|} | |||
==Timecourse Experiment 09/11/2013== | |||
1. Cultured cells from [[Daniel:Notebook/HiResChrPaint/2013-9-9|9/9/13 (step 6)]] (PGP1F, Passage 12) | |||
2. Aspirate media and rinse with 5 mL PBS | |||
3. Aspirate PBS and add 1 mL trypsin solution; incubate for several minutes | |||
4. Add 9 mL DMEM to cell culture, mix by gently pipetting | |||
5. Add 100 uL cell suspension to center of surface (2x slides, 2x dishes) | |||
6. Took time course photos of slides at 3 hours and 6 hours | |||
7. Continued [[Daniel:Notebook/HiResChrPaint/2013-9-12|tomorrow]] |
Latest revision as of 23:51, 11 September 2013
Fibroblast Adherence Timecourse (Started 09/09/2013)[edit]
Petri dishes are in! So I've got a revised itinerary. First, a recap. There are a few important variables to track in this experiment:
- (1) surface type (slide or dish)
- (2) trypsin dilution (to ensure cells may attach)
- (3) Cell seeding density (1,000,000 cells/mL recommended)
- (4) Adding more media to help growth
New Template for Experiment
Name | Surface | Trypsin Dilution | Cell Seeding Density | Media Kicker |
Slide 09/09/13 | Slide | 1:10 | 1.00E+06 | None |
Slide 1 | Slide | 1:20 | 1.00E+06 | None |
Slide 2 | Slide | 1:20 | 1.00E+06 | 100 uL DMEM |
Dish 1 | Dish | 1:20 | 1.00E+06 | None |
Dish 2 | Dish | 1:20 | 1.00E+06 | 100 uL DMEM |
Timecourse Experiment 09/11/2013[edit]
1. Cultured cells from 9/9/13 (step 6) (PGP1F, Passage 12) 2. Aspirate media and rinse with 5 mL PBS 3. Aspirate PBS and add 1 mL trypsin solution; incubate for several minutes 4. Add 9 mL DMEM to cell culture, mix by gently pipetting 5. Add 100 uL cell suspension to center of surface (2x slides, 2x dishes) 6. Took time course photos of slides at 3 hours and 6 hours 7. Continued tomorrow