EricChu:LabNotesMDA/2013-9-15: Difference between revisions

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>Ericchu
(Created page with "== MDA Device v9 (PGP1 #16) with Tagmentation == * Device preparation was described in EricChu:LabNotesMDA/2013-5-20 * MDA recipe was followed to the protocol in EricChu:LabN...")
 
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* 240mM KOH final mixing concentration was used to lyze cell and denature DNA
* 240mM KOH final mixing concentration was used to lyze cell and denature DNA
===ALS===
===ALS===
#H2O       76.78ul
{| {{table}}
#2M DTT     5ul
|H2O
#5M KOH     5.72ul
||
8% Tween20 12.5ul
|76.78 uL
|-
|2M DTT
||
|5 uL
|-
|5M KOH
||
|5.72 uL
|-
|8% Tween20
||
|12.5 uL
|}


===NS===
===NS===
#H2O       24.94ul
 
#NS stock   62.56ul
{| {{table}}
8% Tween20 12.5ul
|H2O
||
|24.94 uL
|-
|NS stock
||
|62.56 uL
|-
|8% Tween20
||
|12.5 uL
|}

Revision as of 01:31, 16 September 2013

MDA Device v9 (PGP1 #16) with Tagmentation

  • Device preparation was described in EricChu:LabNotesMDA/2013-5-20
  • MDA recipe was followed to the protocol in EricChu:LabNotesMDA/2013-5-14
  • Compare experiments of (1) v7 device with Tagmentation and (2) v9 device with Tagmentation

ALS and NS in MDA Device v9

  • 240mM KOH final mixing concentration was used to lyze cell and denature DNA

ALS

H2O 76.78 uL
2M DTT 5 uL
5M KOH 5.72 uL
8% Tween20 12.5 uL

NS

H2O 24.94 uL
NS stock 62.56 uL
8% Tween20 12.5 uL