EricChu:LabNotesMDA/2013-9-15: Difference between revisions
Jump to navigation
Jump to search
>Ericchu (Created page with "== MDA Device v9 (PGP1 #16) with Tagmentation == * Device preparation was described in EricChu:LabNotesMDA/2013-5-20 * MDA recipe was followed to the protocol in EricChu:LabN...") |
>Ericchu No edit summary |
||
Line 8: | Line 8: | ||
* 240mM KOH final mixing concentration was used to lyze cell and denature DNA | * 240mM KOH final mixing concentration was used to lyze cell and denature DNA | ||
===ALS=== | ===ALS=== | ||
{| {{table}} | |||
|H2O | |||
|| | |||
8% Tween20 | |76.78 uL | ||
|- | |||
|2M DTT | |||
|| | |||
|5 uL | |||
|- | |||
|5M KOH | |||
|| | |||
|5.72 uL | |||
|- | |||
|8% Tween20 | |||
|| | |||
|12.5 uL | |||
|} | |||
===NS=== | ===NS=== | ||
{| {{table}} | |||
8% Tween20 | |H2O | ||
|| | |||
|24.94 uL | |||
|- | |||
|NS stock | |||
|| | |||
|62.56 uL | |||
|- | |||
|8% Tween20 | |||
|| | |||
|12.5 uL | |||
|} |
Revision as of 01:31, 16 September 2013
MDA Device v9 (PGP1 #16) with Tagmentation
- Device preparation was described in EricChu:LabNotesMDA/2013-5-20
- MDA recipe was followed to the protocol in EricChu:LabNotesMDA/2013-5-14
- Compare experiments of (1) v7 device with Tagmentation and (2) v9 device with Tagmentation
ALS and NS in MDA Device v9
- 240mM KOH final mixing concentration was used to lyze cell and denature DNA
ALS
H2O | 76.78 uL | |
2M DTT | 5 uL | |
5M KOH | 5.72 uL | |
8% Tween20 | 12.5 uL |
NS
H2O | 24.94 uL | |
NS stock | 62.56 uL | |
8% Tween20 | 12.5 uL |