Daniel:Notebook/HiResChrPaint/2013-9-18: Difference between revisions

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[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]


Did some research this morning on fibroblast culture conditions.  I found an interesting [[http://genome.ucsc.edu/ENCODE/protocols/cell/human/FibroP_Crawford_protocol.pdf|protocol]] on UCSC Genome Browser that seems to be a simple (and useful) protocol for culture.  The protocol recommends four ingredients in culture medium:
Given the bad results from [[Daniel:Notebook/HiResChrPaint/2013-9-16|Monday]] and [[Daniel:Notebook/HiResChrPaint/2013-9-17|Tuesday]], I think it is time to put a little extra thought in to cell culture conditions.  Did some research this morning on fibroblast culture conditions.  I found an interesting [[http://genome.ucsc.edu/ENCODE/protocols/cell/human/FibroP_Crawford_protocol.pdf|protocol]] on UCSC Genome Browser that seems to be a simple (and useful) protocol for culture.  The protocol recommends four ingredients in culture medium:
*DMEM (duh, provides all the essential nutrients)
*DMEM (duh, provides all the essential nutrients)
*Fetal Bovine Serum (has a good deal of growth factors plus lots of BSA)
*Fetal Bovine Serum (has a good deal of growth factors plus lots of BSA)

Revision as of 16:32, 18 September 2013

Fibroblast Adherence (Started 09/09/2013)

Back to Calendar

Given the bad results from Monday and Tuesday, I think it is time to put a little extra thought in to cell culture conditions. Did some research this morning on fibroblast culture conditions. I found an interesting [[1]] on UCSC Genome Browser that seems to be a simple (and useful) protocol for culture. The protocol recommends four ingredients in culture medium:

  • DMEM (duh, provides all the essential nutrients)
  • Fetal Bovine Serum (has a good deal of growth factors plus lots of BSA)
  • 2-Mercaptoethanol (reduces disulfide bonds and acts as an antioxidant)
  • L-glutamine (essential amino acid frequently added to culture)

I think we can skip the L-glutamine, and probably the 2-mercaptoethanol as well. However, I think the fetal bovine serum might help out the cell culture, and as it happens Song has recently ordered some, so I'll ask him about using it.