Song Chen:Daily/NOTES/2013-9-19: Difference between revisions
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*3*100 mm MEF(P2) --> 30 vials | *3*100 mm MEF(P2) --> 30 vials | ||
*estimated | *estimated number of plated 60 mm dishes each vial: 3*10<sup>7</sup>(each 100 mm dish)/30(vials)/5*10<sup>5</sup>= 2 |
Revision as of 00:06, 20 September 2013
Aliquot Mitomycin C(Roche Cat.#10107409001)
- dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 degree
Prepare Mitomycin-C-inactivated MEFs
- ...
- 3*100 mm MEF(P2) --> 30 vials
- estimated number of plated 60 mm dishes each vial: 3*107(each 100 mm dish)/30(vials)/5*105= 2