Song Chen:Daily/NOTES/2013-9-19: Difference between revisions
Jump to navigation
Jump to search
>Chensong No edit summary |
>Chensong No edit summary |
||
Line 4: | Line 4: | ||
Prepare Mitomycin-C-inactivated MEFs | Prepare Mitomycin-C-inactivated MEFs | ||
*[[Song:MEF_Preparation]] | *[[Song:MEF_Preparation] MEF] | ||
*3*100 mm MEF(P2) --> 30 vials | *3*100 mm MEF(P2) --> 30 vials | ||
*estimated number of plated 60 mm dishes each vial: 3*10<sup>7</sup>(each 100 mm dish)/30(vials)/5*10<sup>5</sup>= 2 | *estimated number of plated 60 mm dishes each vial: 3*10<sup>7</sup>(each 100 mm dish)/30(vials)/5*10<sup>5</sup>= 2 |
Revision as of 00:29, 20 September 2013
Aliquot Mitomycin C(Roche Cat.#10107409001)
- dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 degree
Prepare Mitomycin-C-inactivated MEFs
- [[Song:MEF_Preparation] MEF]
- 3*100 mm MEF(P2) --> 30 vials
- estimated number of plated 60 mm dishes each vial: 3*107(each 100 mm dish)/30(vials)/5*105= 2