Song Chen:Daily/NOTES/2013-9-19: Difference between revisions

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Prepare Mitomycin-C-inactivated MEFs
Prepare Mitomycin-C-inactivated MEFs
*[[Song:MEF_Preparation]]
*[[Song:MEF_Preparation|prepare MEF]]
*3*100 mm MEF(P2) --> 30 vials
*3*100 mm MEF(P2) --> 30 vials
*estimated number of plated 60 mm dishes each vial: 3*10<sup>7</sup>(each 100 mm dish)/30(vials)/5*10<sup>5</sup>= 2
*Estimated number of plated 60 mm dishes each vial: 3*10<sup>7</sup>(each 100 mm dish)/30(vials)/5*10<sup>5</sup>= 2

Revision as of 00:37, 20 September 2013

Aliquot Mitomycin C(Roche Cat.#10107409001)

dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 degree


Prepare Mitomycin-C-inactivated MEFs

  • prepare MEF
  • 3*100 mm MEF(P2) --> 30 vials
  • Estimated number of plated 60 mm dishes each vial: 3*107(each 100 mm dish)/30(vials)/5*105= 2