Song Chen:Daily/NOTES/2013-9-19: Difference between revisions
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Aliquot Mitomycin C(Roche Cat.#10107409001) | Aliquot Mitomycin C(Roche Cat.#10107409001) | ||
: | :Dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 degree | ||
Revision as of 00:39, 20 September 2013
Aliquot Mitomycin C(Roche Cat.#10107409001)
- Dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 degree
Freeze Mitomycin-C-inactivated MEFs
- Prepare MEF
- 3*100 mm MEF(P2) --> 30 vials
- Estimated number of plated 60 mm dishes each vial: 3*107(each 100 mm dish)/30(vials)/5*105= 2