Song Chen:Daily/NOTES/2013-9-19: Difference between revisions

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Aliquot Mitomycin C(Roche Cat.#10107409001)
Aliquot Mitomycin C(Roche Cat.#10107409001)
:dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 degree
:Dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 °C




Prepare Mitomycin-C-inactivated MEFs
Freeze Mitomycin-C-inactivated MEFs
*...
*[[Song:MEF_Preparation|Prepare MEF]]
*3*100 mm MEF(P2) --> 30 vials
*3*100 mm MEF(P2) → 30 vials
*estimated cell density: 3*10<sup>7</sup>(each 100 mm dish)
*Estimated number of plated 60 mm dishes each vial: 3*10<sup>7</sup>(each 100 mm dish)/30(vials)/5*10<sup>5</sup>= 2
<br/>[[Song:Daily_Notebook|Return]]

Latest revision as of 00:10, 3 October 2013

Aliquot Mitomycin C(Roche Cat.#10107409001)

Dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 °C


Freeze Mitomycin-C-inactivated MEFs

  • Prepare MEF
  • 3*100 mm MEF(P2) → 30 vials
  • Estimated number of plated 60 mm dishes each vial: 3*107(each 100 mm dish)/30(vials)/5*105= 2


Return