Song Chen:Daily/NOTES/2013-9-19: Difference between revisions
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Aliquot Mitomycin C(Roche Cat.#10107409001) | Aliquot Mitomycin C(Roche Cat.#10107409001) | ||
: | :Dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 °C | ||
Freeze Mitomycin-C-inactivated MEFs | |||
*[[MEF_Preparation]] | *[[Song:MEF_Preparation|Prepare MEF]] | ||
*3*100 mm MEF(P2) | *3*100 mm MEF(P2) → 30 vials | ||
* | *Estimated number of plated 60 mm dishes each vial: 3*10<sup>7</sup>(each 100 mm dish)/30(vials)/5*10<sup>5</sup>= 2 | ||
<br/>[[Song:Daily_Notebook|Return]] |
Latest revision as of 00:10, 3 October 2013
Aliquot Mitomycin C(Roche Cat.#10107409001)
- Dissolved in DMEM, 10μg/ml (2 mg in 200 ml), 50ml each stored in -20 °C
Freeze Mitomycin-C-inactivated MEFs
- Prepare MEF
- 3*100 mm MEF(P2) → 30 vials
- Estimated number of plated 60 mm dishes each vial: 3*107(each 100 mm dish)/30(vials)/5*105= 2