Arichard:Reagents/ns: Difference between revisions

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>Andrew
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>Andrew
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NS buffer:
NS buffer:
Add 3 mL H20, 3 mL 2M Tris buffer, and 4 mL 1N HCl to 15 mL tube.
Add 3 mL H20, 3 mL 2M Tris-HCl, and 4 mL 1N HCl to 15 mL tube.
 
When Jeff says Tris buffer, he means Tris-HCl. Otherwise, he means Tris base

Latest revision as of 20:19, 20 May 2015

From Jeff Human Microbiome Protocols 2009, first entry:

NS buffer: Add 3 mL H20, 3 mL 2M Tris-HCl, and 4 mL 1N HCl to 15 mL tube.