Song Chen:Daily/NOTES/2013-9-25: Difference between revisions
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*Gelatin coating dishes for HepG2 | *Gelatin coating dishes for HepG2 | ||
*Alice's HUES: Rack #1 Box #8 | *Alice's HUES: Rack #1 Box #8 | ||
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[[Song:Daily_Notebook|Notebook]] | [[Song:Daily_Notebook|Notebook]] |
Revision as of 21:25, 26 September 2013
- Freezing 293 cells: 1*15 cm--> 8*vials(each for 10 cm dish)
- Lentivirus from Blue: 3*lenti-Brainbow #2, 1*lenti-Brainbow #4, 1*lenti-CMV-Bb, 1*lentiGFP, 50-100μl for each well of 6-well plate, ~100μ per tube
- Lentivirus infection: 30μlBb#2 for one well 293 cells(just plated)
- Gelatin coating dishes for HepG2
- Alice's HUES: Rack #1 Box #8