Daniel:Notebook/HiResChrPaint/2013-10-1: Difference between revisions

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>Djacobse
(Created page with "=FISH= Back to Calendar ==Slide Preparation== Prepping slides from yesterday. Since I'm not r...")
 
>Djacobse
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===Protocol===
===Protocol===


*Modified from [[Daniel:Protocols/FISH|standard FISH protocol]]
*Modified from [[Daniel:Protocols/FISH#Slide Preparation|standard FISH protocol]], starting at step 5
 
1. Rinse slides briefly in 1X PBS
2. Fix slides for 5-15 minutes in 4% (v/v) paraformaldehyde in 1X PBS
3. Rinse briefly with 1 mL 1X PBS
4. Incubate for 5 minutes in 1 mL 2X SSCT
5. Incubate for 5 minutes in 1 mL 2X SSCT + 50% (v/v) formamide
6. Aspirate, and add 1mL of 2X SSCT + 50% (v/v) formamide for storage at 4C
7. Slides are typically best used within 1-2 weeks of creation

Revision as of 17:16, 1 October 2013

FISH

Back to Calendar

Slide Preparation

Prepping slides from yesterday. Since I'm not ready to do FISH on them quite yet, I'll just fix them for now.

Protocol

1. Rinse slides briefly in 1X PBS
2. Fix slides for 5-15 minutes in 4% (v/v) paraformaldehyde in 1X PBS
3. Rinse briefly with 1 mL 1X PBS
4. Incubate for 5 minutes in 1 mL 2X SSCT
5. Incubate for 5 minutes in 1 mL 2X SSCT + 50% (v/v) formamide
6. Aspirate, and add 1mL of 2X SSCT + 50% (v/v) formamide for storage at 4C
7. Slides are typically best used within 1-2 weeks of creation