Arichard:Notebook/2013/October: Difference between revisions

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>Andrew
(Created page with "==October, 2013== ===10/01/2013=== * Started MIDAS with Alz. neu. nuc. ID #25-00+ ===10/02/2013=== * Forgot to turn on temperature control. Overnight MDA ran at ~22 degC i...")
 
>Andrew
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[[File:2013_10_03_midas_alz_pcr2.jpg|400px]]
[[File:2013_10_03_midas_alz_pcr2.jpg|400px]]
* Chris started a new MIDAS run
===10/04/2013===
* Alan asked me to pool some of my libraries. I gave them new library names afterwards:
# AR-Alz-28-Sep17 + AR-Alz-29-Sep17 + AR-Alz-31a-Sep17 --> AR-Alz-28,29,31-Sep30
# AR-Alz-31b-Sep17 --> AR-Alz-31b-Sep17 (No change, want to sequence separately)
# AR-Alz-30,27-Sep17 + AR-Alz-26,28a-Sep20 --> AR-Alz-26,27,28,30-Sep30
# AR-Alz-28,29-Sep17 + AR-Alz-25,30-Sep20 --> AR-Alz-25,28,29,30-Sep30
# AR-Alz-29,30-Sep20 + AR-Alz-26,28b-Sep20 --> AR-Alz-26,28,29,30-Sep30

Revision as of 00:05, 6 October 2013

October, 2013

10/01/2013

  • Started MIDAS with Alz. neu. nuc. ID #25-00+

10/02/2013

  • Forgot to turn on temperature control. Overnight MDA ran at ~22 degC instead of 30.
    • According to NEB website, Phi29 pol processes 2280 nt/min at 30 degC, and 1490 nt/min at 15 degC.
    • Based on this, I decided to continue with the experiment.
  • Spilled the tubes after adding 75% EtOH wash after precipitation.
    • Tubes 9-12 were closed already, should not have been affected.
    • Other tubes still had visible pellets, so I just added more wash and continued.

10/03/2013

File:2013 10 03 midas alz pcr1.jpg

File:2013 10 03 midas alz pcr2.jpg

  • Chris started a new MIDAS run

10/04/2013

  • Alan asked me to pool some of my libraries. I gave them new library names afterwards:
  1. AR-Alz-28-Sep17 + AR-Alz-29-Sep17 + AR-Alz-31a-Sep17 --> AR-Alz-28,29,31-Sep30
  2. AR-Alz-31b-Sep17 --> AR-Alz-31b-Sep17 (No change, want to sequence separately)
  3. AR-Alz-30,27-Sep17 + AR-Alz-26,28a-Sep20 --> AR-Alz-26,27,28,30-Sep30
  4. AR-Alz-28,29-Sep17 + AR-Alz-25,30-Sep20 --> AR-Alz-25,28,29,30-Sep30
  5. AR-Alz-29,30-Sep20 + AR-Alz-26,28b-Sep20 --> AR-Alz-26,28,29,30-Sep30