Daniel:Notebook/HiResChrPaint/2013-10-5: Difference between revisions

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=V6S3 and V4S1 Amplification Amino Primers ([[Daniel:Notebook/HiResChrPaint/2013-10-3|Started 10/03/2013]])=
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
==DpnII Digestion==
3. [[Daniel:Protocols/Qiaquick_Column|Column purification]], elute with 30 uL EB
4. Measure Concentration in nanodrop
5. Check digestion results with gel
===Nanodrop Results===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
| align="center" width="65" height="15" | &nbsp;
| width="65" | V4S1AB
| width="65" | V4S1CD
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ng/uL
| align="center" | 121.8
| align="center" | 131
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ug in 30 uL
| align="center" | 3.7
| align="center" | 3.9
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | ug total
| align="center" | &nbsp;
| align="center" | 7.6
|}
===Gel Results===


[[Image:20131005_DpnIIDigest.jpg|500px]]
[[Image:20131005_DpnIIDigest.jpg|500px]]

Latest revision as of 21:28, 6 October 2013

V6S3 and V4S1 Amplification Amino Primers (Started 10/03/2013)[edit]

Back to Calendar

DpnII Digestion[edit]

3. Column purification, elute with 30 uL EB
4. Measure Concentration in nanodrop
5. Check digestion results with gel

Nanodrop Results[edit]

  V4S1AB V4S1CD
ng/uL 121.8 131
ug in 30 uL 3.7 3.9
ug total   7.6

Gel Results[edit]

File:20131005 DpnIIDigest.jpg