Daniel:Notebook/HiResChrPaint/2013-10-5: Difference between revisions
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=V6S3 and V4S1 Amplification Amino Primers ([[Daniel:Notebook/HiResChrPaint/2013-10-3|Started 10/03/2013]])= | |||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | ||
==DpnII Digestion== | |||
3. [[Daniel:Protocols/Qiaquick_Column|Column purification]], elute with 30 uL EB | |||
4. Measure Concentration in nanodrop | |||
5. Check digestion results with gel | |||
===Nanodrop Results=== | |||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt;font-weight:bold" align="center" | |||
| align="center" width="65" height="15" | | |||
| width="65" | V4S1AB | |||
| width="65" | V4S1CD | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | ng/uL | |||
| align="center" | 121.8 | |||
| align="center" | 131 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | ug in 30 uL | |||
| align="center" | 3.7 | |||
| align="center" | 3.9 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | ug total | |||
| align="center" | | |||
| align="center" | 7.6 | |||
|} | |||
===Gel Results=== | |||
[[Image:20131005_DpnIIDigest.jpg|500px]] | [[Image:20131005_DpnIIDigest.jpg|500px]] |
Latest revision as of 21:28, 6 October 2013
V6S3 and V4S1 Amplification Amino Primers (Started 10/03/2013)[edit]
DpnII Digestion[edit]
3. Column purification, elute with 30 uL EB 4. Measure Concentration in nanodrop 5. Check digestion results with gel
Nanodrop Results[edit]
V4S1AB | V4S1CD | |
ng/uL | 121.8 | 131 |
ug in 30 uL | 3.7 | 3.9 |
ug total | 7.6 |