Daniel:Notebook/HiResChrPaint/2013-10-5: Difference between revisions
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=V6S3 and V4S1 Amplification Amino Primers ([[Daniel:Notebook/HiResChrPaint/2013-10-3|Started 10/03/2013]])= | |||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | [[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | ||
==DpnII Digestion== | |||
3. [[Daniel:Protocols/Qiaquick_Column|Column purification]], elute with 30 uL EB | |||
4. Measure Concentration in nanodrop | |||
5. Check digestion results with gel | |||
===Nanodrop Results=== | ===Nanodrop Results=== |
Latest revision as of 21:28, 6 October 2013
V6S3 and V4S1 Amplification Amino Primers (Started 10/03/2013)[edit]
DpnII Digestion[edit]
3. Column purification, elute with 30 uL EB 4. Measure Concentration in nanodrop 5. Check digestion results with gel
Nanodrop Results[edit]
V4S1AB | V4S1CD | |
ng/uL | 121.8 | 131 |
ug in 30 uL | 3.7 | 3.9 |
ug total | 7.6 |