Daniel:Notebook/HiResChrPaint/2013-10-5: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
No edit summary
>Djacobse
No edit summary
 
Line 1: Line 1:
=V6S3 and V4S1 Amplification Amino Primers ([[Daniel:Notebook/HiResChrPaint/2013-10-3|Started 10/03/2013]])=
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
==DpnII Digestion==
3. [[Daniel:Protocols/Qiaquick_Column|Column purification]], elute with 30 uL EB
4. Measure Concentration in nanodrop
5. Check digestion results with gel


===Nanodrop Results===
===Nanodrop Results===

Latest revision as of 21:28, 6 October 2013

V6S3 and V4S1 Amplification Amino Primers (Started 10/03/2013)[edit]

Back to Calendar

DpnII Digestion[edit]

3. Column purification, elute with 30 uL EB
4. Measure Concentration in nanodrop
5. Check digestion results with gel

Nanodrop Results[edit]

  V4S1AB V4S1CD
ng/uL 121.8 131
ug in 30 uL 3.7 3.9
ug total   7.6

Gel Results[edit]

File:20131005 DpnIIDigest.jpg