Kun:LabNotes/ASE/2007-11-6: Difference between revisions

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(New page: ===Exp. 1 PAGE purification of the eSNP84k probes=== ===Exp. 2 Continue on the second round of probe prep.=== Starting from the ethanol precipitated PCR amplicons from 800ul reactions. ...)
 
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===Exp. 1 PAGE purification of the eSNP84k probes===
===Exp. 1 PAGE purification of the eSNP84k probes===


      Lib #1:  10ng/ul(380nM) x 40ul
      Lib #2:  15ng/ul(580nM) x 40ul
      Lib #3:  5ng/ul(200nM) x 40ul
      Lib #4:  13ng/ul(500nM) x 40ul
      Lib #5:  12ng/ul(460nM) x 40ul


===Exp. 2 Continue on the second round of probe prep.===
===Exp. 2 Continue on the second round of probe prep.===

Revision as of 00:16, 8 November 2007

Exp. 1 PAGE purification of the eSNP84k probes

     Lib #1:  10ng/ul(380nM) x 40ul 
     Lib #2:  15ng/ul(580nM) x 40ul
     Lib #3:   5ng/ul(200nM) x 40ul
     Lib #4:  13ng/ul(500nM) x 40ul
     Lib #5:  12ng/ul(460nM) x 40ul

Exp. 2 Continue on the second round of probe prep.

Starting from the ethanol precipitated PCR amplicons from 800ul reactions.

The DNAs were resuspended in 100ul H2O. I added 10ul 10X NEBuffer 4, 4ul BciVI, 37C 3h, 75C 10min. I have one extra tube of Lib #5, which was included in the experiment.