Kun:LabNotes/ASE/2007-11-6: Difference between revisions
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(New page: ===Exp. 1 PAGE purification of the eSNP84k probes=== ===Exp. 2 Continue on the second round of probe prep.=== Starting from the ethanol precipitated PCR amplicons from 800ul reactions. ...) |
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===Exp. 1 PAGE purification of the eSNP84k probes=== | ===Exp. 1 PAGE purification of the eSNP84k probes=== | ||
Lib #1: 10ng/ul(380nM) x 40ul | |||
Lib #2: 15ng/ul(580nM) x 40ul | |||
Lib #3: 5ng/ul(200nM) x 40ul | |||
Lib #4: 13ng/ul(500nM) x 40ul | |||
Lib #5: 12ng/ul(460nM) x 40ul | |||
===Exp. 2 Continue on the second round of probe prep.=== | ===Exp. 2 Continue on the second round of probe prep.=== |
Revision as of 00:16, 8 November 2007
Exp. 1 PAGE purification of the eSNP84k probes
Lib #1: 10ng/ul(380nM) x 40ul Lib #2: 15ng/ul(580nM) x 40ul Lib #3: 5ng/ul(200nM) x 40ul Lib #4: 13ng/ul(500nM) x 40ul Lib #5: 12ng/ul(460nM) x 40ul
Exp. 2 Continue on the second round of probe prep.
Starting from the ethanol precipitated PCR amplicons from 800ul reactions.
The DNAs were resuspended in 100ul H2O. I added 10ul 10X NEBuffer 4, 4ul BciVI, 37C 3h, 75C 10min. I have one extra tube of Lib #5, which was included in the experiment.