Song Chen:Daily/NOTES/2013-10-16: Difference between revisions
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*Place:[https://humanstemcellcore.ucsd.edu/index.html UCSD hESC Core] | *Place:[https://humanstemcellcore.ucsd.edu/index.html UCSD hESC Core] | ||
*Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2) | *Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2) | ||
*Brief procedure | |||
:Dissociate culture into single cells | |||
:Wash twice with PBS | |||
:Filter cells with cell strainer | |||
:Suspend cells best in 10<sub>6</sub>/ml density | |||
:Place cell suspension in ice(3rd floor) | |||
:Bring culture medium to collect sorted cells | |||
<br/><b>[[Song:Daily_Notebook|Return]]</b> | <br/><b>[[Song:Daily_Notebook|Return]]</b> |
Revision as of 21:25, 17 October 2013
FACS sorting
- Place:UCSD hESC Core
- Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
- Brief procedure
- Dissociate culture into single cells
- Wash twice with PBS
- Filter cells with cell strainer
- Suspend cells best in 106/ml density
- Place cell suspension in ice(3rd floor)
- Bring culture medium to collect sorted cells