Song Chen:Daily/NOTES/2013-10-16: Difference between revisions

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*Place:[https://humanstemcellcore.ucsd.edu/index.html UCSD hESC Core]
*Place:[https://humanstemcellcore.ucsd.edu/index.html UCSD hESC Core]
*Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
*Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
*Brief procedure
:Dissociate culture into single cells
:Wash twice with PBS
:Filter cells with cell strainer
:Suspend cells best in 10<sub>6</sub>/ml density
:Place cell suspension in ice(3rd floor)
:Bring culture medium to collect sorted cells


<br/><b>[[Song:Daily_Notebook|Return]]</b>
<br/><b>[[Song:Daily_Notebook|Return]]</b>

Revision as of 21:25, 17 October 2013

FACS sorting

  • Place:UCSD hESC Core
  • Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
  • Brief procedure
Dissociate culture into single cells
Wash twice with PBS
Filter cells with cell strainer
Suspend cells best in 106/ml density
Place cell suspension in ice(3rd floor)
Bring culture medium to collect sorted cells


Return