Song Chen:Daily/NOTES/2013-10-16: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Chensong
No edit summary
>Chensong
No edit summary
Line 3: Line 3:
*Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
*Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
*Brief procedure:
*Brief procedure:
:#Dissociate culture into single cells
#Dissociate culture into single cells
:#Wash twice with PBS
#Wash twice with PBS
:Filter cells with cell strainer
#Filter cells with cell strainer
:Suspend cells best in 10<sub>6</sub>/ml density
:Suspend cells best in 10<sub>6</sub>/ml density
:Place cell suspension in ice(3rd floor)
:Place cell suspension in ice(3rd floor)

Revision as of 21:28, 17 October 2013

FACS sorting

  • Place:UCSD hESC Core
  • Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
  • Brief procedure:
  1. Dissociate culture into single cells
  2. Wash twice with PBS
  3. Filter cells with cell strainer
Suspend cells best in 106/ml density
Place cell suspension in ice(3rd floor)
Bring culture medium to collect sorted cells


Return