Song Chen:Daily/NOTES/2013-10-16: Difference between revisions
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#Filter cells with cell strainer | #Filter cells with cell strainer | ||
#Suspend cells best in 10<sup>6</sup>/ml density | #Suspend cells best in 10<sup>6</sup>/ml density | ||
#Place cell suspension in ice(3rd floor) | #Place cell suspension in ice (3rd floor) | ||
#Bring culture medium to collect sorted cells | #Bring culture medium to collect sorted cells | ||
<br/><b>[[Song:Daily_Notebook|Return]]</b> | <br/><b>[[Song:Daily_Notebook|Return]]</b> |
Revision as of 06:28, 19 October 2013
FACS sorting
- Place:UCSD hESC Core
- Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
- Brief procedure:
- Dissociate culture into single cells
- Wash twice with PBS
- Filter cells with cell strainer
- Suspend cells best in 106/ml density
- Place cell suspension in ice (3rd floor)
- Bring culture medium to collect sorted cells