Song Chen:Daily/NOTES/2013-10-16: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Chensong
No edit summary
>Chensong
No edit summary
Line 7: Line 7:
#Filter cells with cell strainer
#Filter cells with cell strainer
#Suspend cells best in 10<sup>6</sup>/ml density
#Suspend cells best in 10<sup>6</sup>/ml density
#Place cell suspension in ice(3rd floor)
#Place cell suspension in ice (3rd floor)
#Bring culture medium to collect sorted cells
#Bring culture medium to collect sorted cells


<br/><b>[[Song:Daily_Notebook|Return]]</b>
<br/><b>[[Song:Daily_Notebook|Return]]</b>

Revision as of 06:28, 19 October 2013

FACS sorting

  • Place:UCSD hESC Core
  • Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
  • Brief procedure:
  1. Dissociate culture into single cells
  2. Wash twice with PBS
  3. Filter cells with cell strainer
  4. Suspend cells best in 106/ml density
  5. Place cell suspension in ice (3rd floor)
  6. Bring culture medium to collect sorted cells


Return