Song Chen:Daily/NOTES/2013-10-16: Difference between revisions
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>Chensong (Created page with "==FACS sorting== https://humanstemcellcore.ucsd.edu/schedule/index.php <br/><b>Return</b>") |
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==FACS sorting== | ==FACS sorting== | ||
[ | *Place:[https://humanstemcellcore.ucsd.edu/index.html UCSD hESC Core] | ||
*Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2) | |||
*Brief procedure: | |||
#Dissociate culture into single cells | |||
#Wash twice with PBS | |||
#Filter cells with cell strainer | |||
#Suspend cells best in the density of 10<sup>6</sup>/ml | |||
#Snap cap of FACS tube to avoid contamination | |||
#Place cell suspension in ice (3rd floor) | |||
#Bring culture medium to collect sorted cells | |||
<br/><b>[[Song:Daily_Notebook|Return]]</b> | <br/><b>[[Song:Daily_Notebook|Return]]</b> |
Latest revision as of 06:33, 19 October 2013
FACS sorting[edit]
- Place:UCSD hESC Core
- Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
- Brief procedure:
- Dissociate culture into single cells
- Wash twice with PBS
- Filter cells with cell strainer
- Suspend cells best in the density of 106/ml
- Snap cap of FACS tube to avoid contamination
- Place cell suspension in ice (3rd floor)
- Bring culture medium to collect sorted cells