Song Chen:Daily/NOTES/2013-10-16: Difference between revisions

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==FACS sorting==
==FACS sorting==
[[hES|https://humanstemcellcore.ucsd.edu/schedule/index.php]]
*Place:[https://humanstemcellcore.ucsd.edu/index.html UCSD hESC Core]
*Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
*Brief procedure:
#Dissociate culture into single cells
#Wash twice with PBS
#Filter cells with cell strainer
#Suspend cells best in the density of 10<sup>6</sup>/ml
#Snap cap of FACS tube to avoid contamination
#Place cell suspension in ice (3rd floor)
#Bring culture medium to collect sorted cells


<br/><b>[[Song:Daily_Notebook|Return]]</b>
<br/><b>[[Song:Daily_Notebook|Return]]</b>

Latest revision as of 06:33, 19 October 2013

FACS sorting[edit]

  • Place:UCSD hESC Core
  • Type: Sorting(BDInflux, FACSAria 2); Analysis(BDLSRFortessa, FACSCanto 2)
  • Brief procedure:
  1. Dissociate culture into single cells
  2. Wash twice with PBS
  3. Filter cells with cell strainer
  4. Suspend cells best in the density of 106/ml
  5. Snap cap of FACS tube to avoid contamination
  6. Place cell suspension in ice (3rd floor)
  7. Bring culture medium to collect sorted cells


Return