Hosuk:LabNotes/2013-10-21: Difference between revisions

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>Hosuki78
(Created page with "*LabNote ===Fixing cells in 96 well plate=== *I've split cells in 16 wells. *First 8 wells are fixed with 2 step, others with 1 step fixing. *For 1 stp...")
 
>Hosuki78
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*For 1 stpe fixing
*For 1 stpe fixing
** Take out 150uL media, so there is 150uL in well
** Take out 150uL media, so there is 150uL in well.
** Add 100uL 10% formalin, so it is 4% formalin in well.
** Add 100uL 10% formalin, so it is 4% formalin in well.




*For 2 step fixing
*For 2 step fixing
** Take out 100uL media, so there is 200#uL in well
** Take out 100uL media, so there is 200#uL in well.
** Add 20uL 37% formalin, so it is 1:10 ratio, as following the first rolony protocol that I've got.
** Add 20uL 37% formalin, so it is 1:10 ratio, as following the first rolony protocol that I've got.
*4 wells in 1st, 2nd row --> 2 step fixing
*4 wells in 3rd, 4th row --> 1 step fixing
*4 wells in 1st, 3rd row --> Anaspec's a-dUTP
*4 wells in 2nd, 4th row --> Invitrogen's a-dUTP

Latest revision as of 04:38, 22 October 2013

Fixing cells in 96 well plate[edit]

  • I've split cells in 16 wells.
  • First 8 wells are fixed with 2 step, others with 1 step fixing.


  • For 1 stpe fixing
    • Take out 150uL media, so there is 150uL in well.
    • Add 100uL 10% formalin, so it is 4% formalin in well.


  • For 2 step fixing
    • Take out 100uL media, so there is 200#uL in well.
    • Add 20uL 37% formalin, so it is 1:10 ratio, as following the first rolony protocol that I've got.


  • 4 wells in 1st, 2nd row --> 2 step fixing
  • 4 wells in 3rd, 4th row --> 1 step fixing
  • 4 wells in 1st, 3rd row --> Anaspec's a-dUTP
  • 4 wells in 2nd, 4th row --> Invitrogen's a-dUTP