Hosuk:LabNotes/2013-10-21: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===Fixing cells in 96 well plate=== *I've split cells in 16 wells. *First 8 wells are fixed with 2 step, others with 1 step fixing. *For 1 stp...") |
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*For 1 stpe fixing | *For 1 stpe fixing | ||
** Take out 150uL media, so there is 150uL in well | ** Take out 150uL media, so there is 150uL in well. | ||
** Add 100uL 10% formalin, so it is 4% formalin in well. | ** Add 100uL 10% formalin, so it is 4% formalin in well. | ||
*For 2 step fixing | *For 2 step fixing | ||
** Take out 100uL media, so there is 200#uL in well | ** Take out 100uL media, so there is 200#uL in well. | ||
** Add 20uL 37% formalin, so it is 1:10 ratio, as following the first rolony protocol that I've got. | ** Add 20uL 37% formalin, so it is 1:10 ratio, as following the first rolony protocol that I've got. | ||
*4 wells in 1st, 2nd row --> 2 step fixing | |||
*4 wells in 3rd, 4th row --> 1 step fixing | |||
*4 wells in 1st, 3rd row --> Anaspec's a-dUTP | |||
*4 wells in 2nd, 4th row --> Invitrogen's a-dUTP |
Latest revision as of 04:38, 22 October 2013
Fixing cells in 96 well plate[edit]
- I've split cells in 16 wells.
- First 8 wells are fixed with 2 step, others with 1 step fixing.
- For 1 stpe fixing
- Take out 150uL media, so there is 150uL in well.
- Add 100uL 10% formalin, so it is 4% formalin in well.
- For 2 step fixing
- Take out 100uL media, so there is 200#uL in well.
- Add 20uL 37% formalin, so it is 1:10 ratio, as following the first rolony protocol that I've got.
- 4 wells in 1st, 2nd row --> 2 step fixing
- 4 wells in 3rd, 4th row --> 1 step fixing
- 4 wells in 1st, 3rd row --> Anaspec's a-dUTP
- 4 wells in 2nd, 4th row --> Invitrogen's a-dUTP