Daniel:Notebook/HiResChrPaint/2013-10-22: Difference between revisions
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>Djacobse (→qPCR) |
>Djacobse |
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{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | ||
|- style="font-size:12pt;font-weight:bold" align="center" | |||
| width="65" height="15" | Sample | |||
| width="65" | BSPP 1A | |||
| width="65" | BSPP 1B | |||
| width="65" | BSPP 1C | |||
| width="65" | BSPP 1D | |||
| width="65" | BSPP 2A | |||
| width="65" | BSPP 2B | |||
| width="65" | BSPP 2C | |||
| width="65" | BSPP 2D | |||
|- style="font-size:12pt" align="center" | |- style="font-size:12pt" align="center" | ||
|style=" | |style="font-weight:bold" height="15" | ng/uL | ||
| | | align="center" | 97.9 | ||
| | | align="center" | 94.5 | ||
| | | align="center" | 96.9 | ||
| | | align="center" | 91.9 | ||
| | | align="center" | 90.2 | ||
| | | align="center" | 92.5 | ||
| align="center" | 90.7 | |||
| align="center" | 82.4 | |||
|- style=" | |- style="font-size:12pt" align="center" | ||
| height="15" | | |style="font-weight:bold" height="15" | ug in 35 uL | ||
| align="center" | | | align="center" | 3.4 | ||
| align="center" | | | align="center" | 3.3 | ||
| align="center" | | | align="center" | 3.4 | ||
| align="center" | | | align="center" | 3.2 | ||
| align="center" | | | align="center" | 3.2 | ||
| align="center" | | | align="center" | 3.2 | ||
| align="center" | 3.2 | |||
| align="center" | 2.9 | |||
|- style="font-size:12pt" align="center" | |- style="font-size:12pt" align="center" | ||
| height="30" | | |style="font-weight:bold" height="30" | uL 10X buffer | ||
| align="center" | 5 | |||
| align="center" | 5 | |||
| align="center" | 5 | | align="center" | 5 | ||
| align="center" | 5 | | align="center" | 5 | ||
| align="center" | 5 | | align="center" | 5 | ||
| align="center" | 5 | | align="center" | 5 | ||
| align="center" | 5 | | align="center" | 5 | ||
| align="center" | 5 | | align="center" | 5 | ||
| | |- style="font-size:12pt" align="center" | ||
|style="font-weight:bold" height="30" | uL DpnII (50U/uL) | |||
| align="center" | 2 | |||
| align="center" | 2 | |||
| align="center" | 2 | |||
| align="center" | 2 | |||
| align="center" | 2 | |||
| align="center" | 2 | |||
| align="center" | 2 | |||
| align="center" | 2 | |||
|- style="font-size:12pt" align="center" | |||
|style="font-weight:bold" height="15" | uL nfH2O | |||
| align="center" | 8 | |||
| align="center" | 8 | |||
| align="center" | 8 | |||
| align="center" | 8 | |||
| align="center" | 8 | |||
| align="center" | 8 | |||
| align="center" | 8 | |||
| align="center" | 8 | |||
=== | |- style="font-size:12pt" align="center" | ||
|style="font-weight:bold" height="15" | Total | |||
| align="center" | 50 | |||
| align="center" | 50 | |||
| align="center" | 50 | |||
| align="center" | 50 | |||
| align="center" | 50 | |||
| align="center" | 50 | |||
| align="center" | 50 | |||
| align="center" | 50 | |||
|} | |||
[[ | 2. Incubate samples at 37C for 16 hours; 20 minute heat shock at 65C; Continued [[Daniel:Notebook/HiResChrPaint/2013-10-23|tomorrow]] |
Revision as of 01:05, 23 October 2013
BSPP Control
- For first production of BSPP control, see 09/03/2013
Since I'm out of control group, I'm going to make some more.
qPCR
1. Reaction Table
Sample | BSPP 1A | BSPP 1B | BSPP 1C | BSPP 1D | BSPP 2A | BSPP 2B | BSPP 2C | BSPP 2D |
ng/uL | 97.9 | 94.5 | 96.9 | 91.9 | 90.2 | 92.5 | 90.7 | 82.4 |
ug in 35 uL | 3.4 | 3.3 | 3.4 | 3.2 | 3.2 | 3.2 | 3.2 | 2.9 |
uL 10X buffer | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
uL DpnII (50U/uL) | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
uL nfH2O | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 |
Total | 50 | 50 | 50 | 50 | 50 | 50 | 50 | 50 |
2. Incubate samples at 37C for 16 hours; 20 minute heat shock at 65C; Continued tomorrow