AlanFung:LabNotes/Rolony/2013-11-14: Difference between revisions

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==Optimum Probe# Test==  
==Optimum Probe# Test==
*In this experiment we are trying to find the concentration of probes that works best for capturing rolony targets. From previous experiments we know that a concentration of 0.6uM with a volume of 100ul works.
*A test of 5 different concentrations will be perform and image analysis using PISA will be performed to count the number of target being captured


==Concentrations==
==Concentrations==

Revision as of 21:37, 14 November 2013

Optimum Probe# Test

  • In this experiment we are trying to find the concentration of probes that works best for capturing rolony targets. From previous experiments we know that a concentration of 0.6uM with a volume of 100ul works.
  • A test of 5 different concentrations will be perform and image analysis using PISA will be performed to count the number of target being captured

Concentrations

5uM
100nM
10nM
1nM
0.1nM