AlanFung:LabNotes/Rolony/2013-11-14: Difference between revisions

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  MALAT1 padlock probes
  MALAT1 padlock probes
  MALAT1 padlock probes detection
  MALAT1 padlock probes detection
===Apparatus===
*warm up incubator to 50C for incubation
*warm up tube heater to 80C for pre-heating padlock probes
===Stripping===
===Stripping===
===Annealing===
===Imaging===

Revision as of 21:42, 14 November 2013

Optimum Probe# Test

  • In this experiment we are trying to find the concentration of probes that works best for capturing rolony targets. From previous experiments we know that a concentration of 0.6uM with a volume of 100ul works.
  • A test of 5 different concentrations will be perform and image analysis using PISA will be performed to count the number of target being captured

Concentrations

5uM
100nM
10nM
1nM
0.1nM

Protocol

  • In this experiment we will be using the Mattek dish containing primary rolonies made on Oct 23rd dish #2

Reagents

80% Formamide in 2X SSC
MALAT1 padlock probes
MALAT1 padlock probes detection

Apparatus

  • warm up incubator to 50C for incubation
  • warm up tube heater to 80C for pre-heating padlock probes

Stripping

Annealing

Imaging