Daniel:Notebook/HiResChrPaint/2013-11-16: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
>Djacobse
 
(2 intermediate revisions by the same user not shown)
Line 35: Line 35:
| align="center" | 25
| align="center" | 25
| align="center" | 25
| align="center" | 25
|


|- style="font-size:12pt" align="center"
|- style="font-size:12pt" align="center"
Line 49: Line 50:
| align="center" | 3
| align="center" | 3
| align="center" | 3
| align="center" | 3
|


|- style="font-size:12pt" align="center"
|- style="font-size:12pt" align="center"
Line 70: Line 72:
  4. [[Daniel:Protocols/ssDNA Column|ssDNA column]] purification, elute with 20 uL nfH2O
  4. [[Daniel:Protocols/ssDNA Column|ssDNA column]] purification, elute with 20 uL nfH2O
  5. Measure Concentration in Nanodrop
  5. Measure Concentration in Nanodrop
===ssNanodrop Results===
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center"
| width="65" height="15" | Sample
| width="65" | ng/uL
| width="65" | ug in 20 uL
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | V6S3
| align="center" | 144.3
| align="center" | 2.9
|- style="font-size:12pt" align="center"
|style="font-weight:bold" height="15" | V6S4
| align="center" | 137.2
| align="center" | 2.7
|}

Latest revision as of 01:41, 17 November 2013

Positive Control 2 (V6S3 & V6S4, Started 11/15/2013)[edit]

Back to Calendar

Lambda Exonuclease Digestion[edit]

1. Reaction table
Sample V6S3AB V6S3CD V6S4AB V6S4CD
ng/uL 169.9 180 169.4 180.1
ug in 25 uL 4.2 4.5 4.2 4.5
uL added 25 25 25 25
uL 10x buffer 5 5 5 5
uL Lambda Exonuclease (5U/uL) 3 3 3 3
uL nfH2O 17 17 17 17
total 50 50 50 50
2. Incubate for 1 hour at 37C followed by 10 minute heat inactivation at 75C
3. Pool samples
4. ssDNA column purification, elute with 20 uL nfH2O
5. Measure Concentration in Nanodrop

ssNanodrop Results[edit]

Sample ng/uL ug in 20 uL
V6S3 144.3 2.9
V6S4 137.2 2.7