Hosuk:LabNotes/2013-11-19: Difference between revisions

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(Created page with "*LabNote ===Dye labeled Probe Annealing Test – with Cy3-1st Rolony=== *Figure out upper limit by Formamide concentration ====Procedure==== *2048 x 2-...")
 
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===Dye labeled Probe Annealing Test – with Cy3-1st Rolony===
===Dye labeled Probe Annealing Test – with Cy3-1st Rolony===
*Figure out upper limit by Formamide concentration
*Figure out upper limit by Formamide concentration
*Done @ 11/18





Revision as of 05:06, 19 November 2013


Dye labeled Probe Annealing Test – with Cy3-1st Rolony

  • Figure out upper limit by Formamide concentration
  • Done @ 11/18


Procedure

  • 2048 x 2-48, 600Hz  Image quality is not different from 400Hz.
  • Sample : MatTek Dish, made at 07/09
  • Each stripping, and annealing : 15min incubation @ RT


  • Process Steps
    • Step 0 : Strip
    • Step 1 : Cy5 in 2x SSC
    • Step 2 : Strip
    • Step 3 : Cy5 in 70% Formamide + 2x SSC
    • Step 4 : Strip
    • Step 5 : Cy5 in 10% Formamide + 2x SSC
    • Step 6 : Strip
    • Step 7 : Cy5 in 30 % Formamide + 2x SSC
    • Step 8 : Strip
    • Step 9 : Cy5 in 50% Formamide + 2x SSC
    • Step 10 : Strip
    • Step 11 : Cy5 in 70% Formamide + 2x SSC


Result