Hosuk:LabNotes/2013-11-19: Difference between revisions
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>Hosuki78 (Created page with "*LabNote ===Dye labeled Probe Annealing Test – with Cy3-1st Rolony=== *Figure out upper limit by Formamide concentration ====Procedure==== *2048 x 2-...") |
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===Dye labeled Probe Annealing Test – with Cy3-1st Rolony=== | ===Dye labeled Probe Annealing Test – with Cy3-1st Rolony=== | ||
*Figure out upper limit by Formamide concentration | *Figure out upper limit by Formamide concentration | ||
*Done @ 11/18 | |||
Revision as of 05:06, 19 November 2013
Dye labeled Probe Annealing Test – with Cy3-1st Rolony
- Figure out upper limit by Formamide concentration
- Done @ 11/18
Procedure
- 2048 x 2-48, 600Hz Image quality is not different from 400Hz.
- Sample : MatTek Dish, made at 07/09
- Each stripping, and annealing : 15min incubation @ RT
- Process Steps
- Step 0 : Strip
- Step 1 : Cy5 in 2x SSC
- Step 2 : Strip
- Step 3 : Cy5 in 70% Formamide + 2x SSC
- Step 4 : Strip
- Step 5 : Cy5 in 10% Formamide + 2x SSC
- Step 6 : Strip
- Step 7 : Cy5 in 30 % Formamide + 2x SSC
- Step 8 : Strip
- Step 9 : Cy5 in 50% Formamide + 2x SSC
- Step 10 : Strip
- Step 11 : Cy5 in 70% Formamide + 2x SSC