Kun:LabNotes/CpgSeq/2013-11-24: Difference between revisions

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(Created page with "==Probe design for the NICE project== *Dinh went through one round of design using ppDesigner v1.1. However, upon close inspection, I found that some capturing arms contain lo...")
 
 
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==Probe design for the NICE project==
 
*Dinh went through one round of design using ppDesigner v1.1. However, upon close inspection, I found that some capturing arms contain long stretches of poly-Ts or poly-Gs.
*I modified get_probes.pl, and filtered out any candidate capturing arm that has over 6 A/T/G/Cs.
*I repeated the probe design.
**Set I: gap size = 400-450bp, flanking len = 250bp
**Set II: gap size = 125-175bp, flanking len = 125bp

Latest revision as of 03:33, 19 March 2014