Arichard:Notebook/2014/January: Difference between revisions

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>Andrew
(Created page with "==January, 2014== ===01/01/2014=== Of the nine bulk libraries (3 timepoints, 3 brains), only 5 indexes contained reads. This corresponds to the gel image. I asked Alan to tr...")
 
>Andrew
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Of the nine bulk libraries (3 timepoints, 3 brains), only 5 indexes contained reads. This corresponds to the gel image. I asked Alan to try to sequence them just in case we could get a few reads, since I don't need very many.
Of the nine bulk libraries (3 timepoints, 3 brains), only 5 indexes contained reads. This corresponds to the gel image. I asked Alan to try to sequence them just in case we could get a few reads, since I don't need very many.
The libraries sequenced were as follows:
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''AR-bulk-25,27-Dec11'''
|-
| AR-bulk-26-Dec11
|-
| AR-bulk-28,30-Dec11
|-
| AR-bulk-29-Dec11
|-
| AR-bulk-31,36-Dec11
|-
| AR-bulk-32-Dec11
|-
| AR-bulk-37,38-Dec11
|}
Index specific info:


{| {{table}}
{| {{table}}
Line 37: Line 57:
|-
|-
| NTC||Neg ctl||HL162||3||38||284868||||||
| NTC||Neg ctl||HL162||3||38||284868||||||
|-
|
|}
Also in HL162, Alan sequenced the rest of the Alzheimer's libraries and the bulk, MDA amplified, Covaris prepped libraries from October.
From AR-Alz-26,28,29,30-Sep30:
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Run'''
| align="center" style="background:#f0f0f0;"|'''Lane'''
| align="center" style="background:#f0f0f0;"|'''NxtIndx'''
| align="center" style="background:#f0f0f0;"|'''Reads'''
|-
| HL162||1||26||579859
|-
| HL162||1||28||1298887
|-
| HL162||1||29||811001
|-
| HL162||1||30||353131
|-
|
|}
From AR-MDA-ILMN-Oct24:
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Run'''
| align="center" style="background:#f0f0f0;"|'''Lane'''
| align="center" style="background:#f0f0f0;"|'''NEBIndx'''
| align="center" style="background:#f0f0f0;"|'''Reads'''
|-
| HL162||1||3||847838
|-
| HL162||1||4||781967
|-
|-
|  
|  
|}
|}

Revision as of 00:46, 2 January 2014

January, 2014

01/01/2014

Of the nine bulk libraries (3 timepoints, 3 brains), only 5 indexes contained reads. This corresponds to the gel image. I asked Alan to try to sequence them just in case we could get a few reads, since I don't need very many.

The libraries sequenced were as follows:

AR-bulk-25,27-Dec11
AR-bulk-26-Dec11
AR-bulk-28,30-Dec11
AR-bulk-29-Dec11
AR-bulk-31,36-Dec11
AR-bulk-32-Dec11
AR-bulk-37,38-Dec11

Index specific info:

Brain Timepoint Run Lane NxtIndx # # Reads ' Timepoint Description
1568+ A HL162 1 25 8460 A Before lysis
1568+ B HL162 3 26 254995 B Before FACS
1568+ C HL162 1 27 65108 C After FACS
1864+ A HL162 3 28 NA
1864+ B HL162 3 29 121356
1864+ C HL162 3 30 NA
61788+ A HL162 3 31 NA
61788+ B HL162 3 32 NA
61788+ C HL162 3 36 NA
5 ng E. coli Pos ctl HL162 3 37 2636700
NTC Neg ctl HL162 3 38 284868

Also in HL162, Alan sequenced the rest of the Alzheimer's libraries and the bulk, MDA amplified, Covaris prepped libraries from October.

From AR-Alz-26,28,29,30-Sep30:

Run Lane NxtIndx Reads
HL162 1 26 579859
HL162 1 28 1298887
HL162 1 29 811001
HL162 1 30 353131

From AR-MDA-ILMN-Oct24:

Run Lane NEBIndx Reads
HL162 1 3 847838
HL162 1 4 781967