Arichard:Notebook/2014/January: Difference between revisions
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>Andrew |
>Andrew |
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Of the | Of the eleven bulk libraries (3 timepoints for 3 brains plus positive and negative control), only 6 indexes contained reads. This corresponds to the gel image. I asked Alan to try to sequence them anyways just in case we could get a few reads, since I don't need very many. | ||
The libraries sequenced were as follows: | The libraries sequenced were as follows: |
Revision as of 23:05, 2 January 2014
January, 2014
01/01/2014
Of the eleven bulk libraries (3 timepoints for 3 brains plus positive and negative control), only 6 indexes contained reads. This corresponds to the gel image. I asked Alan to try to sequence them anyways just in case we could get a few reads, since I don't need very many.
The libraries sequenced were as follows:
AR-bulk-25,27-Dec11 |
AR-bulk-26-Dec11 |
AR-bulk-28,30-Dec11 |
AR-bulk-29-Dec11 |
AR-bulk-31,36-Dec11 |
AR-bulk-32-Dec11 |
AR-bulk-37,38-Dec11 |
Index specific info:
Brain | Timepoint | Run | Lane | NxtIndx # | # Reads | ' | Timepoint | Description |
1568+ | A | HL162 | 1 | 25 | 8460 | A | Before lysis | |
1568+ | B | HL162 | 3 | 26 | 254995 | B | Before FACS | |
1568+ | C | HL162 | 1 | 27 | 65108 | C | After FACS | |
1864+ | A | HL162 | 3 | 28 | NA | |||
1864+ | B | HL162 | 3 | 29 | 121356 | |||
1864+ | C | HL162 | 3 | 30 | NA | |||
61788+ | A | HL162 | 3 | 31 | NA | |||
61788+ | B | HL162 | 3 | 32 | NA | |||
61788+ | C | HL162 | 3 | 36 | NA | |||
5 ng E. coli | Pos ctl | HL162 | 3 | 37 | 2636700 | |||
NTC | Neg ctl | HL162 | 3 | 38 | 284868 | |||
Also in HL162, Alan sequenced the rest of the Alzheimer's libraries and the bulk, MDA amplified, Covaris prepped libraries from October.
From AR-Alz-26,28,29,30-Sep30:
Run | Lane | NxtIndx | Reads |
HL162 | 1 | 26 | 579859 |
HL162 | 1 | 28 | 1298887 |
HL162 | 1 | 29 | 811001 |
HL162 | 1 | 30 | 353131 |
From AR-MDA-ILMN-Oct24:
Run | Lane | NEBIndx | Reads |
HL162 | 1 | 3 | 847838 |
HL162 | 1 | 4 | 781967 |