Daniel:Notebook/HiResChrPaint/2014-1-13: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
(Created page with "=Alu Control (Started 1/08/2014)= Back to Calendar ==Dye Coupling Test== *Following [[Daniel:Pr...")
 
>Djacobse
Line 8: Line 8:


'''Samples'''
'''Samples'''
*[[Daniel:Notebook/HiResChrPaint/2014-1-9#Nanodrop Results|ALU2-AB]]; total 1.1 ug
*[[Daniel:Notebook/HiResChrPaint/2014-1-9#Nanodrop Results|ALU2-C]]; total 1.0 ug
*Human Cot-1 DNA, 1 ug
*Human Cot-1 DNA, 1 ug


Line 22: Line 22:
  8. [[Daniel:Protocols/CentriSep|Centri-Sep column purification]]
  8. [[Daniel:Protocols/CentriSep|Centri-Sep column purification]]
  9. Measure concentration in Nanodrop
  9. Measure concentration in Nanodrop


===Labeling Results===
===Labeling Results===

Revision as of 20:46, 13 January 2014

Alu Control (Started 1/08/2014)

Back to Calendar

Dye Coupling Test

Samples

  • ALU2-C; total 1.0 ug
  • Human Cot-1 DNA, 1 ug
0. Vaccuum centrifuge DNA; resuspend in 2.5 uL nfH2O
1. Warm DMSO and bicarbonate labeling buffer and nuclease free water to room temperature
2. Add 1.5 uL of sodium bicarbonate buffer
3. Denature samples for 5 minutes at 95C, then snap cool using ice box
4. Dissolve 1 vial of reactive dye in 2 uL solvent; vortex ≥10 seconds
   *dye cannot be saved for later use.  Use immediately!
5. Add 1 uL dye to sample
6. Incubate in the dark for 1 hour
7. Add 20 uL nfH20 to sample; Measure concentration in Nanodrop
8. Centri-Sep column purification
9. Measure concentration in Nanodrop

Labeling Results