AlanFung:LabNotes/Capturing/2014-1-14: Difference between revisions

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>Alan6017518
(Created page with "==Capturing Bisulfite Converted Jurkat gDNA w GP1 V6 probe set==")
 
>Alan6017518
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==Capturing Bisulfite Converted Jurkat gDNA w GP1 V6 probe set==
==Capturing Bisulfite Converted Jurkat gDNA w GP1 V6 probe set==
==Capturing Jurkat gDNA w ppMALT1==
==Bisulfite Conversion of Jurkat gDNA==
*Bisulfite conversion with zymo methylation gold
*Convert 500ng of Jurkat gDNA
*Add 130ul of lighting conversion reagent to 5ul of jurkat gDNA and 15ul of ddH2O
*Mix and centrifuge
1. 98°C for 8 minutes
2. 54°C for 60 minutes
3. 4°C storage for up to 20 hours

Revision as of 19:34, 14 January 2014

Capturing Bisulfite Converted Jurkat gDNA w GP1 V6 probe set

Capturing Jurkat gDNA w ppMALT1

Bisulfite Conversion of Jurkat gDNA

  • Bisulfite conversion with zymo methylation gold
  • Convert 500ng of Jurkat gDNA
  • Add 130ul of lighting conversion reagent to 5ul of jurkat gDNA and 15ul of ddH2O
  • Mix and centrifuge
1. 98°C for 8 minutes 
2. 54°C for 60 minutes 
3. 4°C storage for up to 20 hours