AlanFung:LabNotes/Capturing/2014-1-14: Difference between revisions
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>Alan6017518 (Created page with "==Capturing Bisulfite Converted Jurkat gDNA w GP1 V6 probe set==") |
>Alan6017518 |
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==Capturing Bisulfite Converted Jurkat gDNA w GP1 V6 probe set== | ==Capturing Bisulfite Converted Jurkat gDNA w GP1 V6 probe set== | ||
==Capturing Jurkat gDNA w ppMALT1== | |||
==Bisulfite Conversion of Jurkat gDNA== | |||
*Bisulfite conversion with zymo methylation gold | |||
*Convert 500ng of Jurkat gDNA | |||
*Add 130ul of lighting conversion reagent to 5ul of jurkat gDNA and 15ul of ddH2O | |||
*Mix and centrifuge | |||
1. 98°C for 8 minutes | |||
2. 54°C for 60 minutes | |||
3. 4°C storage for up to 20 hours |
Revision as of 19:34, 14 January 2014
Capturing Bisulfite Converted Jurkat gDNA w GP1 V6 probe set
Capturing Jurkat gDNA w ppMALT1
Bisulfite Conversion of Jurkat gDNA
- Bisulfite conversion with zymo methylation gold
- Convert 500ng of Jurkat gDNA
- Add 130ul of lighting conversion reagent to 5ul of jurkat gDNA and 15ul of ddH2O
- Mix and centrifuge
1. 98°C for 8 minutes 2. 54°C for 60 minutes 3. 4°C storage for up to 20 hours