AlanFung:Lab Notebook/DNA Purification: Difference between revisions

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Conclusion:With 260/280 ratio close to 2 there seems to be RNA contaminate. In order to obtain RNA-free gDNA prior to step 2 in the protocol 4uL RNase A (100mg/ml) should be added.
Conclusion:With 260/280 ratio close to 2 there seems to be RNA contaminate. In order to obtain RNA-free gDNA prior to step 2 in the protocol 4uL RNase A (100mg/ml) should be added.
==Test==

Latest revision as of 22:45, 4 August 2013

Date: 10/10/08

== DNeasy Experiment==

Purpose: For purification of total DNA from GC1F fibroblasts cells & PGP1F cells

Material & Protocol:Media:DNeasy.pdf

Notes

-2samples: GC1F and PGP1F(red capped 15mL tube) -Broke down cell pellet by pipetting -Without trypsin EDTA, thawed cells from frozen vials. Start from step 2: lysis on own protocol


Results:

GC1F(Made in Oct.2008) gDNA 36.4ng/uL 260/280: 2.01

PGP1F (Made in Sep. 2008) gDNA: 163.4ng/uL 260/280: 2.08

Both gDNA vials stored in Box 1 in -70freezer located in Kun Lab.

Conclusion:With 260/280 ratio close to 2 there seems to be RNA contaminate. In order to obtain RNA-free gDNA prior to step 2 in the protocol 4uL RNase A (100mg/ml) should be added.